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  • Dept. of Reprogramming Science
  • Shinya Yamanaka
  • Yasuhiro Yamada
  • Hirohide Saito
  • Knut Woltjen
  • Yoshinori Yoshida
  • Shinji Masui
  • Masato Nakagawa
  • Keisuke Okita
  • Kazutoshi Takahashi
  • Takuya Yamamoto
  • Akitsu Hotta

  

  

  • Dept. of Cell Growth and Differentiation
  • Junya Toguchida
  • Noriyuki Tsumaki
  • Jun K. Yamashita
  • Wataru Fujibuchi
  • Shin Kaneko
  • Kenji Osafune
  • Makoto Ikeya

  

  

  • Dept. of Clinical Application
  • Tatsutoshi Nakahata
  • Jun Takahashi
  • Koji Eto
  • Yoshiya Kawaguchi
  • Haruhisa Inoue
  • Megumu Saito
  • Hidetoshi Sakurai

  

  

  • Dept. of Fundamental Cell Technology
  • Takafumi Kimura
  • Isao Asaka

  

  

  • Uehiro Research Div. for iPS Cell of Ethics
  • Misao Fujita
  • Yoshimi Yashiro

  
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• Research Overview
• Curriculum Vitae
• Publications
• Members

Publications

Sasagawa, S., Takemori, H., Uebi, T., Ikegami, D., Hiramatsu, K., Ikegawa, S., Yoshikawa, H. and Tsumaki, N

SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice Development, in press

Hiramatsu, K., Sasagawa, S., Outani, H., Nakagawa, K., Yoshikawa, H. and Tsumaki, N.

Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors. J. Clin. Invest., 121: 640-57, 2011.

Ikegami, D., Akiyama, H., Suzuki, A., Nakamura, T., Nakano, T., Yoshikawa, H. and Tsumaki, N

Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development, 138: 1507-19, 2011.

Okamoto, M., Murai, J., Imai, Y., Ikegami, D., Kamiya, N., Kato, S., Mishina, Y., Yoshikawa, H. and Tsumaki, N

Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice. J. Bone Miner. Res., 26: 2511-22, 2011.

Ikegami, D., Iwai, T., Ryo, S., Gu, N., Sugiyama, T., Oh, I., Yoshikawa, H. and Tsumaki, N

Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage Osteoarthritis Cartilage, 19: 233-41, 2011.

Outani, H., Okada, M., Hiramatsu, K., Yoshikawa, H. and Tsumaki, N

Induction of chondrogenic cells from dermal fibroblast culture by defined factors does not involve a pluripotent state. Biochem. Biophys. Res. Commun., 411: 607-12, 2011.

Hiramatsu, K., Iwai, T., Yoshikawa, H. and Tsumaki, N

Expression of dominant negative TGF-beta receptors inhibits cartilage formation in conditional transgenic mice. J. Bone Miner. Metab., 29: 493-500, 2011.

Nakamura, Y., Yamamoto, K., He, X., Otsuki, B., Kim, Y., Murao, H., Soeda, T., Tsumaki, N., Deng, J. M., Zhang, Z., Behringer, R. R., Crombrugghe, B., Postlethwait, J. H., Warman, M. L., Nakamura, T. and Akiyama, H

Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25 Nat Commun, 2: 251, 2011.

Suzuki, D., Yamada, A., Amano, T., Yasuhara, R., Kimura, A., Sakahara, M., Tsumaki, N., Takeda, S., Tamura, M., Nakamura, M., Wada, N., Nohno, T., Shiroishi, T., Aiba, A. and Kamijo, R

Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development. Dev. Biol. 335: 396-406, 2009.

Iwai, T., Murai, J., Yoshikawa, H. and Tsumaki, N.

Smad7 Inhibits chondrocyte differentiation at multiple steps during endochondral bone formation and down-regulates p38 MAPK pathways J. Biol. Chem., 283: 27154-64, 2008.

Murai, J., Ikegami, D., Okamoto, M., Yoshikawa, H. and Tsumaki, N

Insulation of the ubiquitous Rxrb promoter from the cartilage-specific adjacent gene, Col11a2 J. Biol. Chem., 283: 27677-87, 2008.

Nampei, A., Hashimoto, J., Koyanagi, J., Ono, T., Hashimoto, H., Tsumaki, N., Tomita, T., Sugamoto, K., Nishimoto, N., Ochi, T. and Yoshikawa, H.:

Characteristics of fracture and related factors in patients with rheumatoid arthritis. Modern rheumatology / the Japan Rheumatism Association, 18: 170-6, 2008.

Sugiki, T., Uyama, T., Toyoda, M., Morioka, H., Kume, S., Miyado, K., Matsumoto, K., Saito, H., Tsumaki, N., Takahashi, Y., Toyama, Y. and Umezawa, A.

Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts. J. Cell. Biochem., 100: 1240-54, 2007.

Iwai, T., Harada, Y., Imura, K., Iwabuchi, S., Murai, J., Hiramatsu, K., Myoui, A., Yoshikawa, H. and Tsumaki, N.

Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite ceramic J. Bone Miner. Metab., 25: 392-9, 2007.

Okamoto, M., Murai, J., Yoshikawa, H. and Tsumaki, N

Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts during bone development. J. Bone Miner. Res., 21: 1022-33, 2006.

Hirao, M., Tamai, N., Tsumaki, N., Yoshikawa, H. and Myoui, A

Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification. J. Biol. Chem., 281: 31079-92, 2006.

Tsumaki, N., Kakiuchi, M., Sasaki, J., Ochi, T. and Yoshikawa, H

Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with opening-wedge high tibial osteotomy by hemicallotasis J. Bone Joint Surg. Am., 86-A: 2399-405, 2004.

Horiki, M., Imamura, T., Okamoto, M., Hayashi, M., Murai, J., Myoui, A., Ochi, T., Miyazono, K., Yoshikawa, H. and Tsumaki, N

Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes dwarfism with osteopenia. J. Cell Biol., 165: 433-45, 2004.

Katayama, R., Wakitani, S., Tsumaki, N., Morita, Y., Matsushita, I., Gejo, R. and Kimura, T

Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous mesenchymal cells derived from bone marrow Rheumatology (Oxford). 43: 980-5, 2004.

Matsui, Y., Chansky, H. A., Barahmand-Pour, F., Zielinska-Kwiatkowska, A., Tsumaki, N., Myoui, A., Yoshikawa, H., Yang, L. and Eyre, D. R

COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG J. Biol. Chem., 278: 11369-75, 2003

Tsumaki, N., Nakase, T., Miyaji, T., Kakiuchi, M., Kimura, T., Ochi, T. and Yoshikawa, H

Bone morphogenetic protein signals are required for cartilage formation and differently regulate joint development during skeletogenesis J. Bone Miner. Res., 17: 898-906, 2002.

Nakase, T., Sugamoto, K., Miyamoto, T., Tsumaki, N., Luyten, F. P., Inui, H., Myoui, A., Tomita, T. and Yoshikawa, H

Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff. Clin Orthop Relat Res: 140-5, 2002

Matsui, Y., Araki, N., Tsuboi, H., Tsumaki, N., Nakata, K., Kawabata, H., Eyre, D. R. and Yoshikawa, H

Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells Clin Orthop Relat Res: 140-5, 2002

Nakase, T., Ariga, K., Yonenobu, K., Tsumaki, N., Luyten, F. P., Mukai, Y., Sato, I. and Yoshikawa, H

Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum Eur. Spine J., 10: 289-94, 2001.

Matsui, Y., Nakata, K., Araki, N., Ozono, K., Fujita, Y., Tsumaki, N., Kawabata, H., Yasui, N., Sekiguchi, K. and Yoshikawa, H

Alternative splicing of fibronectin transcripts in osteochondrogenic tumors Anticancer Res., 21: 1103-6, 2001

Matsui, Y., Yasui, N., Kawabata, H., Ozono, K., Nakata, K., Mizushima, T., Tsumaki, N., Kataoka, E., Fujita, Y. and Ochi, T

A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal chondrodysplasia. J. Hum. Genet., 45: 105-8, 2000.

Liu, Y., Li, H., Tanaka, K., Tsumaki, N. and Yamada, Y.

Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha2(XI) collagen gene J. Biol. Chem., 275: 12712-8, 2000.

Tsumaki, N., Tanaka, K., Arikawa-Hirasawa, E., Nakase, T., Kimura, T., Thomas, J. T., Ochi, T., Luyten, F. P. and Yamada, Y.

Role of CDMP-1 in skeletal morphogenesis: promotion of mesenchymal cell recruitment and chondrocyte differentiation. J. Cell Biol., 144: 161-73, 1999

Tsumaki, N., Kimura, T., Tanaka, K., Kimura, J. H., Ochi, T. and Yamada, Y.

Modular arrangement of cartilage- and neural tissue-specific cis-elements in the mouse alpha2(XI) collagen promoter. J. Biol. Chem., 273: 22861-4, 1998.

Sugimoto, M., Kimura, T., Tsumaki, N., Matsui, Y., Nakata, K., Kawahata, H., Yasui, N., Kitamura, Y., Nomura, S. and Ochi, T

Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing mouse Cell Tissue Res., 292: 325-32, 1998.

Matsui, Y., Yasui, N., Kimura, T., Tsumaki, N., Kawabata, H. and Ochi, T.

Genotype phenotype correlation in achondroplasia and hypochondroplasia. J. Bone Joint Surg. Br., 80: 1052-6, 1998.

Matsui, Y., Kimura, T., Tsumaki, N., Nakata, K., Yasui, N., Araki, N., Hashimoto, N., Uchida, A. and Ochi, T

Splicing patterns of type XI collagen transcripts act as molecular markers for osteochondrogenic tumors Cancer Lett., 124: 143-8, 1998.

Tsumaki, N., Kimura, T., Matsui, Y., Nakata, K. and Ochi, T.

Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI) collagen gene expression in the embryonic mouse cartilage. J. Cell Biol., 134: 1573-82, 1996.

Tsumaki, N., Sugimoto, M., Nakata, K., Matsui, Y., Ochi, T. and Kimura, T.

Separable regulatory elements from pro-alpha 2 (XI) collagen gene drive distinct patterns of cartilage-specific expression in transgenic mice. Ann. N. Y. Acad. Sci., 785: 340-2, 1996.

Matsui, Y., Kimura, T., Tsumaki, N., Yasui, N. and Ochi, T.

A recurrent 1992delCT mutation of the type X collagen gene in a Japanese patient with Schmid metaphyseal chondrodysplasia. Jpn. J. Hum. Genet., 41: 339-42, 1996.

Kimura, T., Nakata, K., Tsumaki, N., Miyamoto, S., Matsui, Y., Ebara, S. and Ochi, T.

Progressive degeneration of articular cartilage and intervertebral discs. An experimental study in transgenic mice bearing a type IX collagen mutation Int. Orthop., 20: 177-81, 1996

Iwasaki, M., Okada, K., Tsumaki, N., Obata, H., Shirasaki, N. and Oka, S

Cervical spondylotic radiculopathy involving two adjacent nerve roots. Anterior decompression through a single level intervertebral approach. Int. Orthop., 20: 137-41, 1996.

Tsumaki, N. and Kimura, T.

Differential expression of an acidic domain in the amino-terminal propeptide of mouse pro-alpha 2(XI) collagen by complex alternative splicing. J. Biol. Chem., 270: 2372-8, 1995.

Books

Yoshikawa H, Tsumaki N, Myoui A

Chapter 14 Bone Biology: Development and Regeneration Mechanisms, in Physiological and Pathological Conditions, In Strategies in Regenerative Medicine, 2009, Matteo Santin editor, Springer, ISBN 978-0-387-74659-3

Tsumaki N, Liu Y, Yamada Y, and Krebsbach P

Enhancer analysis of the a1(II) and a2(XI) collagen genes in transfected chondrocytes and transgenic mice. In Streuli, C. H., and Grant, M. E. (eds.) Extracellular Matrix Protocols, Methods Mol Biol, 139, Humana press, Totowa, NJ, USA, pp. 187-195, 2000.

Kimura T, Nakata K, Tsumaki N, Ono K

Altered chondrocyte gene expression in articular cartilage matrix components and susceptibility to cartilage destruction. In Mow, V.C. (Ed.) Cell Mechanics and Cellular Engineering, Springer-Verlag, New York, USA, pp. 445-456, 1994.

Review

Tsumaki, N.*, and Yoshikawa, H

The role of bone morphogenetic proteins in endochondral bone formation Cytokine Growth Factor Rev 16:279-285, 2005.