Research Activities

Research Activities

Home › Research Activities › Principal Investigators › Knut Woltjen (Associate Professor)

Principal Investigators

Dept. of Life Science Frontiers 
Knut Woltjen (Associate Professor)

Publication

Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, Ohnishi M, Sakuma T, Soga T, Yamamoto T and Woltjen K.

Microhomology-assisted scarless genome editing in human iPSCs. Nature Communications, 2018; 9, 939.

Kondo T, Imamura K, Funayama M, Tsukita K, Miyake M, Ohta A, Woltjen K, Nakagawa M, Asada T, Arai T, Kawakatsu S, Izumi Y, Kaji R, Iwata N and Inoue H.

iPSC-Based Compound Screening and in Vitro Trials Identify a Synergistic Anti-Amyloid Β Combination for Alzheimer's Disease. Cell Reports, 2017; 21, 2304-2312.

Kojima Y, Sasaki K, Yokobayashi S, Sakai Y, Nakamura T, Yabuta Y, Nakaki F, Nagaoka S, Woltjen K, Hotta, A, Yamamoto T and Saitou M.

Evolutionarily Distinctive Transcriptional and Signaling Programs Drive Human Germ Cell Lineage Specification From Pluripotent Stem Cells. Cell Stem Cell, 2017; 21, 517-532.e5.

Hirosawa M, Fujita Y, Parr CJC, Hayashi K, Kashida S, Hotta A, Woltjen K and Saito H.

Cell-Type-Specific Genome Editing with a microRNA-Responsive CRISPR-Cas9 Switch. Nucleic Acids Research, 2017; 45, e118.

Imamura K, Izumi Y, Watanabe A, Tsukita K, Woltjen K, Yamamoto T, Hotta A, Kondo T, Kitaoka S, Ohta A, Tanaka A, Watanabe D, Morita M, Takuma H, Tamaoka A, Kunath T, Wray S, Furuya H, Era T, Makioka K, Okamoto K, Fujisawa T, Nishitoh H, Homma K, Ichijo H, Julien JP, Obata N, Hosokawa M, Akiyama H, Kaneko S, Ayaki T, Ito H, Kaji R, Takahashi R, Yamanaka S and Inoue H.

The Src/C-Abl Pathway Is a Potential Therapeutic Target in Amyotrophic Lateral Sclerosis. Science Translational Medicine, 2017; 9 (391).

Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S and Yamamoto T.

Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency. Cell Metabolism, 2017; 25, 1103-1117.e6.

Hashimoto K, Yamada Y, Semi K, Yagi M, Tanaka A, Itakura F, Aoki H, Kunisada T, Woltjen K, Haga H, Sakai Y, Yamamoto T and Yamada Y.

Cellular Context-Dependent Consequences of Apc Mutations on Gene Regulation and Cellular Behavior. Proceedings of the National Academy of Sciences, 2017; 114, 758-763.

Komura S, Semi K, Itakura F, Shibata H, Ohno T, Hotta A, Woltjen K, Yamamoto T, Akiyama H and Yamada Y.

An EWS-FLI1-Induced Osteosarcoma Model Unveiled a Crucial Role of Impaired Osteogenic Differentiation on Osteosarcoma Development. Stem Cell Reports, 2016; 6, 592-606.

Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H and Woltjen K.

Engineering the AAVS1 Locus for Consistent and Scalable Transgene Expression in Human iPSCs and Their Differentiated Derivatives. Methods. 2016 May 15;101:43-55. doi: 10.1016/j.ymeth.2015.12.012. Epub 2015 Dec 18.

Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S, Woltjen K.

Inducible Trangene Expression in Human iPS Cells Usind Versatile All-in-One piggyBac Transposons. Methods in Molecular Biology, 2016;1357:111-31. doi: 10.1007/7651_2015_251.

Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, Yamamoto T, Yamanaka S and Woltjen K.

KLF4 N-terminal variance modulates induced reprogramming to pluripotency. Stem Cell Reports, 2015; 4, 1-17.

Chantzoura E, Skylaki S, Menendez S, Kim SI, Johnsson A, Linnarsson S, Woltjen K, Chambers I and Kaji K.

Reprogramming Roadblocks Are System Dependent. Stem Cell Reports, 2015; 5(3), 350-364.

Sasaki K, Yokobayashi S, Nakamura T, Okamoto I, Yabuta Y, Kurimoto K, Ohta H, Moritoki Y, Iwatani C, Tsuchiya H, Nakamura S, Sekiguchi K, Sakuma T, Yamamoto T, Mori T, Woltjen K, Nakagawa M, Yamamoto T, Takahashi K, Yamanaka S and Saitou M.

Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells. Cell Stem Cell, 2015; 17(2), 178-194.

Jin Y, Elalaf H, Watanabe M, Tamaki S, Hineno S, Matsunaga K, Woltjen K, Kobayashi Y, Nagata S, Ikeya M, Kato T, Okamoto T, Matsuda S and Toguchida J.

Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes. PLoS ONE, 2015; 10(7), e0131998.

Tamaki S, Fukuta M, Sekiguchi K, Jin Y, Nagata S, Hayakawa K, Hineno S, Okamoto T, Watanabe M, Woltjen K, Ikeya M, Kato T and Toguchida J.

SS18-SSX, the Oncogenic Fusion Protein in Synovial Sarcoma, Is a Cellular Context-Dependent Epigenetic Modifier. PLoS ONE, 2015; 10(11), e0142991.

Matsuda M, Koga M, Woltjen K, Nishida E and Ebisuya M.

Synthetic lateral inhibition governs cell-type bifurcation with robust ratios. Nature Communications, 2015; 6, 6195.

Sakuma T and Woltjen K.

Nuclease-mediated genome editing: At the front-line of functional genomics technology. Development, growth and differentiation, 2014; 56, 2-13.

Ohnishi K, Semi K, Yamamoto T, Shimizu M, Tanaka A, Mitsunaga K, Okita K, Osafune K, Arioka Y, Maeda T, Soejima H, Moriwaki H, Yamanaka S, Woltjen K, Yamada Y.

Premature Termination of Reprogramming In Vivo Leads to Cancer Development through Altered Epigenetic Regulation. Cell, 2014; 156(4), 663-677.

Gallagher D, Norman AA, Woodard CL, Yang G, Gauthier-Fisher A, Fujitani M, Vessey JP, Cancino GI, Sachewsky N, Woltjen K, Fatt MP, Morshead CM, Kaplan DR and Miller FD.

Transient maternal IL-6 mediates long-lasting changes in neural stem cell pools by deregulating an endogenous self-renewal pathway. Cell Stem Cell, 2013; 13(5), 564-576.

Yamashita A, Liu S, Woltjen K, Thomas B, Meng G, Hotta A, Takahashi K, Ellis J, Yamanaka S and Rancourt DE.

Cartilage tissue engineering identifies abnormal human induced pluripotent stem cells. Scientific Reports, 2013; 3(1978).

Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E and Sakurai H.

Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro. PLoS One, 2013; 8(4), e61540.

Sakuma T, Hosoi S, Woltjen K, Suzuki KI, Kashiwagi K, Wada H, Ochiai H, Miyamoto T, Kawai N, Sasakura Y, Matsuura S, Okada Y, Kawahara A, Hayashi S and Yamamoto T.

Efficient TALEN construction and evaluation methods for human cell and animal applications. Genes to Cells, 2013; 18(4), 315-326.

Hayakawa K, Ikeya M, Fukuta M, Woltjen K, Tamaki S, Takahara N, Kato T, Sato S, Otsuka T and Toguchida J.

Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells. Biochem. Biophys. Research Communications, 2013; 432(4), 713-719.

Hochedlinger K, Takahashi K, Plath K, Ng HH, Clark A, Martin U, Hanna JH, Deng H, Silva JCR, Woltjen K, Boyer L and Wernig M.

Reprogramming: The Next Generation. Cell Stem Cell, 2012; 11(6), 740-743.

Michael IP, Monetti C, Chiu AC, Zhang P, Baba T, Nishino K, Agha-Mohammadi S, Woltjen K, Sung HK and Nagy A.

Highly efficient site-specific transgenesis in cancer cell lines. Molecular Cancer, 2012; 11(1), 89-100.

Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, Nedelec S, Wichterle H, Woltjen K, Hughes TR, Zandstra P.W, Nagy A, Wrana JL and Blencowe BJ.

An alternative splicing switch regulates embryonic stem cell pluripotency and reprogramming. Cell, 2011; 147(1), 132-146.

Nagy K, Sung H, Zhang P, Laflamme S, Vincent P, Agha-Mohammadi S, Woltjen K, Monetti C, Michael IP, Smith LC and Nagy A.

Induced Pluripotent Stem Cell Lines Derived from Equine Fibroblasts. Stem Cell Reviews and Reports, 2011; doi:10.1007/s12015-011-9239-5

Monetti C, Nishino K, Biechele S, Zhang P, Baba T, Woltjen K and Nagy A.

PhiC31 integrase facilitates genetic approaches combining multiple recombinases. Methods, 2011; 53(4), 380-385.

Samavarchi-Tehrani P, Golipour A, David L, Sung HK, Beyer TA, Datti A, Woltjen K, Nagy A and Wrana JL.

Functional Genomics Reveals a BMP Driven Mesenchymal-to-Epithelial Transition in the Initiation of Somatic Cell Reprogramming. Cell Stem Cell., 2010; 7, 64-77.

Woltjen K, Stanford W.L.

Inhibition of tgf-Beta signaling improves mouse fibroblast reprogramming. Cell Stem Cell, 2009; Vol.5 (5): p457-458.

O'Malley J, Woltjen K, Kaji K.

New strategies to generate induced pluripotent stem cells. Current Opinions in Biotechnology, 2009; Vol.20: p516-521.

Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K.

Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature, 2009; Vol.458 (7239): p771-775.

Woltjen K, Michael I.P, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A.

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature, 2009; Vol.458 (7239): p766-770.

Thomas B, Woltjen K and Rancourt DE.

Deep screening of recombination proficient bacteriophage libraries. Biotechniques, 2003; Vol.34 (1): p36-38, 40.

Aoyama C, Woltjen K, Mansergh FC, Ishidate K and Rancourt DE.

Bacteriophage gene targeting vectors generated by transplacement. Biotechniques, 2002; Vol.33 (4): p806-810, 812.

Woltjen K, Bain G, Rancourt DE.

Retro-recombination screening of a mouse embryonic stem cell genomic library. Nucleic Acids Research, 2002; Vol.28 (9): E41.
Authored Book Chapters

Woltjen K, Yamamoto T, Kokubu C and Takeda J.

Report on the Conference on Transposition and Genome Engineering 2015 (TGE 2015): Advancing Cutting-Edge Genomics Technology in the Ancient City of Nara. Genes to cells, 2015; 392-395.

Kim SI, Oceguera-Yanez F, Sakurai C, Nakagawa M, Yamanaka S and Woltjen K.

Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons. Methods in Molecular Biology, 2015; (Clifton, NJ).

Woltjen K, Kim SI, and Nagy A.

The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse. Methods in Molecular Biology, 2015; (Clifton, NJ).

Shoji E, Woltjen K, and Sakurai H.

Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells. Methods in Molecular Biology, 2015; (Clifton, NJ).

Woltjen K, Hämäläinen R, Kibschull M, Mileikovsky M and Nagy A.

Transgene-free production of pluripotent stem cells using piggyBac transposons. Methods in Molecular Biology, 2011; 767, 87-103.

Sciabica K, Woltjen K, Hotta A.

Multiplex Gene-Expression Assay for Human Induced Pluripotent Stem Cells. Genetic Engineering and Biotechnology News, 2010; Vol.30 (3).

Woltjen K, Ito K, Tsuzuki T, Rancourt DE.

Orpheus recombination: a comprehensive bacteriophage system for murine targeting vector construction by transplacement. Methods in Molecular Biology, 2008; Vol.435: p79-94.

Woltjen K, Unger MW, Rancourt DE

Transplacement mutagenesis. A recombination-based in situ mutagenesis protocol. Methods in Molecular Biology, 2002; Vol.182: p189-207.
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