Embryonic stem cells (ESCs) are established by blastocyst in vitro culture and have an ability to proliferate infinitely with maintaining differentiation ability into three germ layers, named pluripotency. Previously, we identified ECATs (ES Cells Associated Transcripts) as genes which are expressed in undifferentiated ESCs and germ cells but not in somatic cells. Functional analysis revealed [...]
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Induced pluripotent stem cells (iPSC) are generated from mouse and human fibroblasts by the introduction of three transcription factors, namely Oct3/4, Sox2, and Klf4. The protooncogene product c-Myc markedly promotes iPSC generation, but it also increases tumor formation in iPSC-derived chimera mice. We herein show that the promotion of iPSC generation by Myc is independent [...]
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Mouse iPS cells are indistinguishable from ES cells in many aspects and produce germline-competent chimeras. Reactivation of the c-Myc retrovirus, however, results in an increased tumorigenicity in the chimeras and progeny mice, thus hindering clinical applications. In the current study, we developed a modified protocol for the induction of iPS cells, which does not require [...]
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