Naka lab

CiRA, Kyoto Univ.


[共同研究成果] HLA-ホモ接合性iPSCのハプロバンキングのための臍帯血バンクの再利用と複数の集団への有用性

韓国のDr. JIHWAN SONGの仕事がStem Cellsにパブリッシュされました。韓国と日本のHLA型の保有状況は似ているので将来的に相互利用の可能性もあり、重要な論文であると思います。

DOI: 10.1002/stem.2865
Title: Repurposing the Cord Blood Bank for Haplobanking of HLA-Homozygous iPSCs and Their Usefulness to Multiple Populations
Authors: Lee S1, Huh JY2, Turner DM3,4, Lee S1, Robinson J5,6, Stein JE5, Shim SH1, Hong CP7, Kang MS2, Nakagawa M8, Kaneko S8, Nakanishi M9, Rao MS10, Kurtz A11, Stacey GN12, Marsh SGE5,6, Turner ML4,13, Song J1,13.
Although autologous induced pluripotent stem cells (iPSCs) can potentially be useful for treating patients without immune rejection, in reality it will be extremely expensive and labor-intensive to make iPSCs to realize personalized medicine. An alternative approach is to make use of human leukocyte antigen (HLA) haplotype homozygous donors to provide HLA matched iPSC products to significant numbers of patients. To establish a haplobank of iPSCs, we repurposed the cord blood bank by screening $4,200 high resolution HLA typed cord blood samples, and selected those homozygous for the 10 most frequent HLA-A,-B,-DRB1 haplotypes in the Korean population. Following the generation of 10 iPSC lines, we conducted a comprehensive characteri- zation, including morphology, expression of pluripotent markers and cell surface antigens, three- germ layer formation, vector clearance, mycoplasma/microbiological/viral contamination, endo- toxin, and short tandem repeat (STR) assays. Various genomic analyses using microarray and comparative genomic hybridization (aCGH)-based single nucleotide polymorphism (SNP) and copy number variation (CNV) were also conducted. These 10 HLA-homozygous iPSC lines match 41.07% of the Korean population. Comparative analysis of HLA population data shows that they are also of use in other Asian populations, such as Japan, with some limited utility in ethnically diverse populations, such as the UK. Taken together, the generation of the 10 most frequent Korean HLA-homozygous iPSC lines serves as a useful pointer for the development of optimal methods for iPSC generation and quality control and indicates the benefits and limitations of col- laborative HLA driven selection of donors for future stocking of worldwide iPSC haplobanks.