Woltjen Lab

Stem Cells and Genome Engineering

Archive for March, 2015

KLF4 stoichiometry varies between polycistronic constructs

Dr. Shin-Il Kim and his lab mates have been working like machines to unravel a reprogramming mystery. Employing piggyBac transposons to deliver two seemingly similar polycistronic reprogramming cassettes (both employing the Yamanaka Factors Oct3/4, Sox2, Klf4, and c-Myc), achieves two drastically distinct reprogramming outcomes. With one vector, colonies are plentiful, yet ultimately fail to fully […]