Woltjen Lab

Stem Cells and Genome Engineering

Author Archive

Conference on Transposition and Genome Engineering (TGE 2015)

As one of this year’s organizers, I am pleased to announce the Conference on Transposition and Genome Engineering (TGE 2015), which will be held November 17-20, 2015 at the Kasugano International Forum IRAKA, in Nara, Japan. This is an amazing opportunity to join a expert group of scientists (including Emmanuelle Charpentier, Dana Carroll, Keith Joung, […]

New Graduate Student: Harunobu Kagawa

A new Ph.D. Graduate Student, Harunobu Kagawa, has joined our lab from today! Kagawa-San obtained his M.Sc. at Hiroshima University, studying the role of the cell cycle in human iPSC reprogramming processes. Moreover, he is the first Japanese Graduate Student in the Woltjen Lab. πŸ™‚ Welcome to the lab, Haru! ι ‘εΌ΅γ‚ŠγΎγ—γ‚‡γ†οΌ

KLF4 stoichiometry varies between polycistronic constructs

Dr. Shin-Il Kim and his lab mates have been working like machines to unravel a reprogramming mystery. Employing piggyBac transposons to deliver two seemingly similar polycistronic reprogramming cassettes (both employing the Yamanaka Factors Oct3/4, Sox2, Klf4, and c-Myc), achieves two drastically distinct reprogramming outcomes. With one vector, colonies are plentiful, yet ultimately fail to fully […]

Collaborator employs piggyBac for synthetic gene cascade

Collaborator Miki Ebisuya (RIKEN Unit Leader and JST PRESTO member) employed multiple piggyBac transposons to deliver components of the Notch-Delta cascade to mammalian cells without such inherent cell-cell signalling. By recreating the gene network artificially, her research team was able to break cellular symmetry, and generate an asymmetric population of cells in an appropriate ratio. […]

CiRA International Symposium in Full Swing

The 2013 CiRA International Symposium kicked off today with talks from leaders in the stem cell field, including Austin Smith, Konrad Hochedlinger, and 2012 Nobel Prize winner John Gurdon. For the full program please visit the official website. If you couldn’t make it to Kyoto this year, make sure to plan ahead for 2014~!