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Protocols

Protocols on iPS cells

CiRA was established at Kyoto University - the birth place of iPS cell technology. As the world's first institution focusing on iPS cell research, CiRA will help the research community by providing information on iPS cell protocols.


Mouse iPS Cells
[Generation Method of Fbx15- and Nonog-iPS Cells]

Please refer to the following paper:
Induction of pluripotent stem cells from fibroblast cultures,
Nature Protocols 2, 3081 - 3089 ( Nov. 1, 2007)



Please refer to the following paper:
Induction of pluripotent stem cells from fibroblast cultures,
Nature Protocols 2, 3081 - 3089 ( Nov. 1, 2007)



[Improved Generation Method of iPS Cells]

- Without antibiotic selection
- With 3 factors excluding c-Myc

See the protocol(PDF: Ver.2) | Updated: July. 17, 2008



[Generation Method of Mouse iPS Cells with Plasmid Vectors]

Please refer to the following paper: Generation of mouse-induced pluripotent stem cells with plasmid vectors, (Nature protocols VOL.4 NO.12 2009)


Human iPS Cells
[Generation and culture of Human iPS Cells under feeder-free condition]

See the protocol(PDF) | Updated: March 11, 2014


[Generation of Human iPS Cells]

See the protocol(PDF) | Updated: March 4, 2009



[Generation and Characterization of Human iPS Cells]

Please refer to the following paper

"Generation and Characterization of Human Induced Pluripotent Stem Cells"

Mari Ohnuki, Kazutoshi Takahashi and Shinya Yamanaka
Current Protocols in Stem Cell Biology June, 2009



[Generation of Human iPS cell induction from human T lymphocytes]

See the protocol(PDF) | Updated: January 10, 2013



[Generation of Human iPS cell induction from human non-T, B cells from peripheral blood]

See the protocol(PDF) | Updated: January 10, 2013



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