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  • Non–Profit Organizations
  • For–Profit Organizations

  
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Human iPS Cells 409B2 and 454E2 are now available through RIKEN BRC under some conditions.

Kyoto University has made the following iPS cells and relevant materials available to the scientific research community through RIKEN BioResource Center ("RIKEN"), a public repository, for the benefit of scientific progress. The materials will be provided to non-profit academic research institutions solely for teaching and academic research purposes.

[For–Profit Organizations]
Please see the page for "For–Profit Organizations" if you are a researcher at a for–profit organization.

Human iPS Cells

*Human iPS Cells are available through RIKEN BRC. Please contact RIKEN BRC directly at cellbank@brc.riken.jp for more details.

409B2 Okita K, et al. Nat. Methods (2011) 454E2 Okita K, et al. Nat. Methods (2011) 201B7 Takahashi K, et al. Cell. 131(5): 861-72 (2007) 253G1 Nakagawa M, et al. Nat Biotechnol. 26(1): 101-6 (2008)

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Mouse iPS Cells

iPS-MEF-Ng-20D-17 Okita K et al. Nature 448: 313-317 (2007) iPS-MEF-Ng-178B-5 Nakagawa M et al. Nature Biotechnology 26: 101-106 (2008) iPS-MEF-Fb/Ng-440A-3 Okita K et al. Science 322: 949-953 (2008) iPS-MEF-Ng-492B-4 Okita K et al. Science 322: 949-953 (2008) iPS-Stm-FB/gfp-99-1 Aoi et al. Science 321: 699-702 (2008) iPS-Stm-FB/gfp-99-3 Aoi et al. Science 321: 699-702 (2008) iPS-Hep-FB/Ng/gfp-103C-1 Aoi et al. Science 321: 699-702 (2008)

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Mouse

Stock Tg(Nanog-GFP,Puro)1Yam Okita K et al. Nature 448: 313-317 (2007) B6;129S4-Fbxo15<tm1Yam> Tokuzawa, Y. et al. Mol Cell Biol. 23(8): 2699-2708 (2003) B6;129S4-ERas<tm1Yam> Takahashi K et al. Nature. 423(6939): 541-545 (2003) B6;129S4-Nanog<tm1Yam> Mitsui, K. et al. Cell. 113(5): 631-642 (2003) STOCK Tg(CAG-EGFP)1YamFbxo15 <tm1Yam> Takahashi K et al. Cell. 126(4): 663-676. Epub 2006 Aug 10. (2006) Tokuzawa, Y. et al. Mol Cell Biol. (8):2699-2708 (2003)

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B6.129S4-Visfatin <tm1Yam> N/A B6;129S4-Ecat8<tm1Yam> N/A

B6;129S4–Frap1tm1 Murakami M et al. Mol Cell Biol. 24(15): 6710-8 (2004) B6;129S4–Frap1tm2 Murakami M et al. Mol Cell Biol. 24(15): 6710-8 (2004)

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Mouse ES Cells

Fbx15 β geo/β geo ES Tokuzawa, Y. et al. Mol. Cell. Biol. 23: 2699-2708 (2003) Nanog β geo/+ ES Mitsui, K. et al. Cell. 113(5): 631-642 (2003) Nanog β geo/hyg Mitsui, K. et al. Cell. 113(5): 631-642 (2003)

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Plasmid

We deposit more than 100 kinds of plasmid with Addgene, a non-profit plasmid repository.

pCXWB–EBNA1 pCXLE–hGLIS1 pCXLE–gw Okita K, et al. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293 pMXs–GLIS1 pMXs-mGLIS1 Maekawa M, et al. Nature. 2011 Jun; 474(7350): 225-9 pCXLE–h OCT3/4–shp53–F pCXLE–hSK pCXLE–hUL Okita K, et al. N. Methods. 2011May; 8(5):409-12 pMXs-Hu-L-Myc Nakagawa M, et al. PNAS. 2010 Aug 10. 107(32):14152-7 pMXs-gw Takahashi K, et al. Cell. 2006 Aug 25. 126(4):663-76 etc. pMXs–p53 Hong H, et al. Nature. 2009 Aug 27. 460(7259):1132-5 pCX–OKS–2A Okita K, et al. Science. 2008 Nov 7. 322(5903):949–53 Human 4 factors (in pMXs) Takahashi K, et al. Cell. 2007 Nov 30. 131(5):861–72 Mouse 4 factors (in pMXs) Takahashi K, et al. Cell. 2006 Aug 25. 126(4):663–76

Request Plasmid to Addgene

Please contact the researchers or organizations below to obtain the following materials.

DNA

The human Gateway® entry clones (containing transcription factor Glis1 gene)

[ Contact ] Biological Resource Center (NBRC), National Institut of Technology and Evaluation (NITE)

Cells

PlatE packaging cells	[ Contact ] tech*cellbiolabs.com Cell Biolabs, Inc
SNL feeder cells	[ Contact ] ECACC
RF8 ES cells	[ Contact ] Dr. Robert Farese Jr. (bfarese*gladstone.ucsf.edu) Gladstone Institute

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