|Yasuhiro Yamada M.D.,Ph.D.
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Epigenetic abnormalities represented by abnormal DNA methylation accompanied by no abnormal base sequence have been clarified to play an important role in carcinogenesis. Recent studies have clarified that abnormal DNA methylation promotes colorectal carcinogenesis through abnormal cell differentiation. We will attempt the resetting of epigenetic abnormalities of cancer cells employing the iPS cell technique. Firstly, we will modify epigenetic conditions of cancer cells. Changes in biological characteristics of cancer cells following separation of abnormal gene sequences from the epigenetic conditions will be investigated. We will also identify the origin of epigenetic abnormalities in cancer cells by analyzing changes in the modification following forced epigenetic alteration and use the results to develop new "epigenetic cancer therapy.":
Aberrant cell proliferation of carcinoma cells in large intestine (dyed with Ki-67/BrdU)
Aberrant differentiation of carcinoma cells in large intestine (dyed with PAS-AB)