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Home › News and Events › 2022 › Researches › Single-cell RNA sequencing of hiPSC-derived muscle progenitor cells identifies key factors of proliferation

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April 25, 2022

Single-cell RNA sequencing of hiPSC-derived muscle progenitor cells identifies key factors of proliferation

The group led by Dr. Minas Nalbandian has established an atlas of Human pluripotent stem cell-derived muscle progenitor cells (hiPSC-MuPCs) and found the heterogeneity in hiPSC-MuPCs.

The research group has been developing transplantation therapy of hiPSC-MuPCs as a treatment for skeletal muscle diseases such as Duchenne muscular dystrophy (DMD). However, it was unclear whether hiPSC-MuPCs has a uniform cell population or not.

In this study, the group performed single-cell RNA sequencing (scRNA-seq) of hiPSC-MuPCs cultures to study the cell heterogeneity of the myogenic subset of cells and found four clusters of cells: noncycling progenitors, cycling, committed, and myocytes. Furthermore, they found FGFR4 and CD36 to be useful markers for separating these subpopulations and demonstrated that the FGFR4-positive cell population has a higher regenerative capacity.

The findings were published online in the Life Science Alliance on 22 April 2022.

Paper Details
  • Journal: Life Science Alliance
  • Title: Single-cell RNAseq reveals heterogeneity in hiPSC-MuPCs and E2F as a key regulator of proliferation
  • Authors: Minas Nalbandian1,*, Mingming Zhao1, Hiroki Kato1,2, Tatsuya Jonouchi1, May Nakajima-Koyama1, Takuya Yamamoto1 and Hidetoshi Sakurai1,*
  • Author Affiliations:
    1. Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
    2. Asahi Kasei Corporation
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