Protocols

Research Activities

Protocols on iPS cells

CiRA was established at Kyoto University - the birth place of iPS cell technology. As the world's first institution focusing on iPS cell research, CiRA will help the research community by providing information on iPS cell protocols.

Mouse iPS Cells

Generation Method of Fbx15- and Nonog-iPS Cells

Please refer to the following paper:
- Induction of pluripotent stem cells from fibroblast cultures
  Nature Protocols 2 3081 - 3089
  (01 Nov., 2007)
Improved Generation Method of iPS Cells

- Without antibiotic selection
- With 3 factors excluding c-Myc
- See the protocol (Ver.2)
  | Updated: July. 17, 2008
Generation Method of Mouse iPS Cells with Plasmid Vectors

Please refer to the following paper:
- Generation of mouse-induced pluripotent stem cells with plasmid vectors
  Nature Protocols（VOL.4 NO.12 2009）
  
  Nature Protocols（VOL.4 NO.12 2009）

Human iPS Cells

Generation and culture of Human iPS Cells under feeder-free condition

Please refer to the following paper:
- See the protocol ｜ Updated: March 11, 2014
Generation of Human iPS cell induction from human T lymphocytes
- See the protocol ｜ Updated: January 10, 2013
Generation of Human iPS cell induction from human non-T,
B cells from peripheral blood
- See the protocol ｜ Updated: January 10, 2013
Generation of Human iPS Cells
- See the protocol ｜ Updated: March 5, 2009
Generation and Characterization of Human iPS Cells

Please refer to the following paper:
- Generation and Characterization of Human Induced Pluripotent
  Stem Cells
  Mari Ohnuki, Kazutoshi Takahashi and Shinya Yamanaka Current Protocols in
  Stem Cell Biology June, 2009
  
  Mari Ohnuki, Kazutoshi Takahashi and Shinya Yamanaka Current Protocols in Stem Cell Biology June, 2009