私達のグループは、細胞運命を決める分子機構を明らかにすることを目的に研究を行っております。特にシングルセルレベルでの遺伝子発現やエピゲノム変化を解析し、体細胞からiPS細胞、あるいはiPS細胞から心筋や神経細胞をはじめとする種々の細胞への分化過程を描写しております。そして、癌に特有の変異を持つ細胞が、これらの正常な分化から逸脱する瞬間を捉え、創薬への展開を試みております。さらに高次なインシリコ解析によって、細胞運命を決定するネットワークを同定します。
Welcome to Watanabe Laboratory at Department of Life Science Frontiers, CiRA, Kyoto University. Pluripotent stem cells such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) have generated enormous interest in regenerative medicine. Not only for the clinical applications, pluripotent cells have been also utilized to a cell model for understanding the molecular mechanisms of maintenance of cellular identity. Dissection of epigenetic regulation during reprogramming process may describe how cells sustain their cell fate, and provide candidate molecules acting as guardians of differentiation. A current high-performance sequencing technology makes us possible to not only genome sequencing but also single-base resolution methylome analysis by whole genome bisulfite sequencing (WGBS) at once. We aim to catalog epigenetic land scape od iPS cells, and identify epigenome marks, which define cell identity.