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May 23, 2011

CiRA Seminar: Dr. Karl Tryggvason

CiRA will invite Dr. Karl Tryggvason from Karolinska Institute in Sweden as a speaker and hold a seminar targeted to reserachers on May 24th .

CiRA holds a series of seminars at random times, inviting speakers inviting speakers from Japan and abroad. Admission is free and pre-registration is not required.

CiRA Seminar
Speaker Karl Tryggvason, M.D., Ph.D.
Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
Lecture title Laminins as extracellular modulators of cellular behavior : Expansion of pluripotent hES cells from single cell suspensions on laminin matrices
Date / Time May 24th, 16:00 - 17:30
Venue Auditorium at the Center for iPS Cell Research and Application, Kyoto University (Language: English)
Host Center for iPS Cell Research and Application, Kyoto University
Abstract The laminns are a family of large trimeric glycoproteins that exist in at least 15 different chain combinations. Recently we showed that laminin-511, that is expressed by pluripotent human stem (hES) cells, can alone support long-term self-renewal of hES and iPS (hES/iPS) cells in a xeno-free cell culture environment (Rodin et al, Nat. Biotechnol. 2010). However, laminin-511 failed to permit stable survival of the cells after replating from single cell suspension. More recently, we have produced and characterized, recombinant human laminin-521, another laminin isoform expressed by pluripotent hES cells and a part of their natural niche. Laminin-521 was also shown to support self-renewal of hES cells in a completely defined, feeder-free and xeno-free cell culture system using TeSR2TM medium. Importantly, however, this laminin isoform allowed survival and expansion of pluripotent hES cells after plating from single cell suspension, and subsequent long-term self-renewal.
Contact Center for iPS Cell Research and Application
Research Promotion Group, Director's Office
Yoko Miyake
E-mail: y.miyake@cira.kyoto-u.ac.jp
(Please change [at] to @)
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