News and Events
News and Events
May 23, 2011
CiRA Seminar: Dr. Karl Tryggvason
CiRA will invite Dr. Karl Tryggvason from Karolinska Institute in Sweden as a speaker and hold a seminar targeted to reserachers on May 24th .
CiRA holds a series of seminars at random times, inviting speakers inviting speakers from Japan and abroad. Admission is free and pre-registration is not required.
Karl Tryggvason, M.D., Ph.D.
Division of Matrix Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden
|Lecture title||Laminins as extracellular modulators of cellular behavior : Expansion of pluripotent hES cells from single cell suspensions on laminin matrices|
|Date / Time||May 24th, 16:00 - 17:30|
|Venue||Auditorium at the Center for iPS Cell Research and Application, Kyoto University (Language: English)|
|Host||Center for iPS Cell Research and Application, Kyoto University|
|Abstract||The laminns are a family of large trimeric glycoproteins that exist in at least 15 different chain combinations. Recently we showed that laminin-511, that is expressed by pluripotent human stem (hES) cells, can alone support long-term self-renewal of hES and iPS (hES/iPS) cells in a xeno-free cell culture environment (Rodin et al, Nat. Biotechnol. 2010). However, laminin-511 failed to permit stable survival of the cells after replating from single cell suspension. More recently, we have produced and characterized, recombinant human laminin-521, another laminin isoform expressed by pluripotent hES cells and a part of their natural niche. Laminin-521 was also shown to support self-renewal of hES cells in a completely defined, feeder-free and xeno-free cell culture system using TeSR2TM medium. Importantly, however, this laminin isoform allowed survival and expansion of pluripotent hES cells after plating from single cell suspension, and subsequent long-term self-renewal.|
Center for iPS Cell Research and Application
Research Promotion Group, Director's Office
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