Research Activities
Research Activities
Publications
April 25, 2022
Single-cell RNA sequencing of hiPSC-derived muscle progenitor cells identifies key factors of proliferation
The research group has been developing transplantation therapy of hiPSC-MuPCs as a treatment for skeletal muscle diseases such as Duchenne muscular dystrophy (DMD). However, it was unclear whether hiPSC-MuPCs has a uniform cell population or not.
In this study, the group performed single-cell RNA sequencing (scRNA-seq) of hiPSC-MuPCs cultures to study the cell heterogeneity of the myogenic subset of cells and found four clusters of cells: noncycling progenitors, cycling, committed, and myocytes. Furthermore, they found FGFR4 and CD36 to be useful markers for separating these subpopulations and demonstrated that the FGFR4-positive cell population has a higher regenerative capacity.
The findings were published online in the Life Science Alliance on 22 April 2022.
Paper Details
- Journal: Life Science Alliance
- Title: Single-cell RNAseq reveals heterogeneity in hiPSC-MuPCs and E2F as a key regulator of proliferation
- Authors: Minas Nalbandian1,*, Mingming Zhao1, Hiroki Kato1,2, Tatsuya Jonouchi1, May Nakajima-Koyama1, Takuya Yamamoto1 and Hidetoshi Sakurai1,*
- Author Affiliations:
- Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan
- Asahi Kasei Corporation
