A method for preparing iPSC through the introduction of four reprogramming genes (Oct3/4, Sox2, Klf4, c-Myc) into somatic cell.

• Press Release

A method for preparing iPSC through the introduction of three reprogramming genes (Oct3/4, Sox2, Klf4) into somatic cell and the culturing in the presence of bFGF.

• Press Release

A method for preparing somatic cells, which comprise production process for the generation of iPSC generated either through the introduction of three reprogramming genes (Oct3/4, Sox2, Klf4) into somatic cell and the culturing in the presence of bFGF, or through the introduction of four reprogramming genes (Oct3/4, Sox2, Klf4, c-Myc) into somatic cell, and the generation of somatic cell from such iPSC through induced differentiation.

• Press Release

• (A) A method for preparing iPSC through the introduction of specific gene families (an Oct3/4 family , a Klf4 family, a Myc family and a Sox2 family genes) ^*1 into somatic cell.
  (wherein at least one of specific gene family for the introduction into the somatic cell is omissible on the condition that corresponding gene is endogenously expressing gene in the somatic cell.)

• (B) A method for preparing iPSC through the introduction of specific gene families (an Oct3/4 family , a Klf4 family and a Sox2 family genes) ^*1 into somatic cell and culture in the presence of bFGF.
  (wherein at least one of specific gene family for the introduction into the somatic cell is omissible on the condition that corresponding gene is endogenously expressing gene in the somatic cell.)

• (C) The method for differentiating iPS cells that were obtained by the method described in (A) or (B).

• Press Release

• (A) Inducing agent to induce iPS cells in the condition of culturing in the presence of bFGF, comprising the three genes Oct3/4, Klf4, and Sox2 or gene products thereof.

• (B) Inducing agent to induce iPS cells, comprising the four genes Oct3/4, Klf4. Sox2, and c-Myc, or gene products thereof.

A method for producing induced pluripotent stem cells, comprising the following (1) to (3) steps;

• (1) selecting combination of genes whose insertion into somatic cells induce expression of the endogenous Oct3/4 gene and the Nanog gene from among genes displaying specific or high-level expression in ES cells, genes encoding factors activated by Wnt signaling or LIF signaling, genes essential to the maintenance of pluripotency in ES cells, and the family genes thereof,

• (2) introducing the combination of genes selected in step (1) into somatic cells, and

• (3) culturing the cells obtained in step (2).

• Press Release

A method for producing induced pluripotent stem cells, comprising the following (1) to (3) steps;

• (1) selecting combination of genes whose insertion into somatic cells induce expression of the endogenous Oct3/4 gene and the Nanog gene from among genes displaying specific or high-level expression in ES cells, genes encoding factors activated by Wnt signaling or LIF signaling, genes essential to the maintenance of pluripotency in ES cells, and the family genes thereof,

• (2) introducing the combination of genes selected in step (1) into somatic cells, and

• (3) culturing the cells obtained in step (2).

A method for producing induced pluripotent stem cells, comprising the following (1) to (3) steps;

• (1) selecting combination of genes whose insertion into somatic cells induce expression of the endogenous Oct3/4 gene and the Nanog gene from among genes displaying specific or high-level expression in ES cells, genes encoding factors activated by Wnt signaling or LIF signaling, genes essential to the maintenance of pluripotency in ES cells, and the family genes thereof,

• (2) introducing the combination of genes selected in step (1) into somatic cells, and

• (3) culturing the cells obtained in step (2).

A reprogramming factor comprising genes of an Oct family, a Klf family, and a Myc family.
A reprogramming factor comprising genes of an Oct family and a Klf family, and a cytokine.

• Press Release

A method for preparing iPSC through the introduction by retroviral vector(s) comprising four reprogramming genes (Oct3/4, Sox2, Klf4, c-Myc) into somatic cell and the post culturing on a fibroblast feeder layer or an extracellular matrix with ES cell growth condition.

• Press Release

A method for preparing mammalian somatic cell(s) by differentiating iPSC obtained through the use of four reprogramming genes (Oct3/4, Sox2, Klf4, c-Myc) under substantially the same process of above US8,058,065 patent.
A method for preparing mammalian somatic cell(s) by differentiating iPSC obtained through the use of three reprogramming genes (Oct3/4, Sox2, Klf4) under substantially the same process of above US8,058,065 patent.

• Press Release

A method for preparing iPSC by nuclear reprogramming of a mammmalian somatic cell, comprising the steps of introducing into the mammalian somatic cell one or more retroviral vectors comprising reprogramming genes (Oct3/4, Klf4, Sox2) , and culturing the transduced somatic cell in the presence of a cytokine.

• Press Release

A reprogramming factor comprising genes or gene products of an Oct family, a Klf family, and a Myc family.
A reprogramming factor comprising genes or gene products of an Oct family and a Klf family, and a cytokine.

• Press Release

An in vitro method for evaluating the physiological function, efficacy or toxicity of a compound, a medicament or poison on cells differentiated from iPSC in which ① Oct family genes, Klf family genes, Sox family genes, and Myc family genes were transduced and cultured cytokines.

An in vitro method for reprogramming a somatic cell which comprises contacting somatic cells with a nuclear reprograming factor comprising ① Oct family genes, Klf family genes, Sox family genes, and Myc family genes and cultures them with cytokines.