Research Activities

Research Activities

Home › Research Activities › Principal Investigators › Makoto Ikeya (Associate Professor)

Principal Investigators

Dept. of Clinical Application 
Makoto Ikeya (Associate Professor)

Publications

Mitsuzawa S, Zhao C, Ikeguchi R*, Aoyama T, Kamiya D, Ando M, Takeuchi H, Akieda S, Nakayama K, Matsuda S, Ikeya M*.

Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell-derived mesenchymal stem cells promotes peripheral nerve regeneration. Stem Cell Reports. 2020 Jul 21;10(1):12034. doi: 10.1038/s41598-020-68745-1. PMID: 32694698

Zhao M, Tazumi A, Takayama S, Takenaka-Ninagawa N, Nalbandian M, Nagai M, Nakamura Y, Nakasa M, Watanabe A, Ikeya M, Hotta A, Ito Y, Sato T, Sakurai H.

Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model. Stem Cell Reports. 2020 Jun 23. pii: S2213-6711(20)30225-3. doi: 10.1016/j.stemcr.2020.06.004.
[Epub ahead of print] PMID: 32619494

Matsuda M, Yamanaka Y, Uemura M, Osawa M, Saito MK, Nagahashi A, Nishio M, Guo L, Ikegawa S, Sakurai S, Kihara S, Maurissen TL, Nakamura M, Matsumoto T, Yoshitomi H, Ikeya M, Kawakami N, Yamamoto T, Woltjen K, Ebisuya M, Toguchida J, Alev C.

Recapitulating the human segmentation clock with pluripotent stem cells. Nature. 2020 Apr;580(7801):124-129. doi: 10.1038/s41586-020-2144-9.
Epub 2020 Apr 1. PMID: 32238941

Tsujimoto H, Kasahara T, Sueta SI, Araoka T, Sakamoto S, Okada C, Mae SI, Nakajima T, Okamoto N, Taura D, Nasu M, Shimizu T, Ryosaka M, Li Z, Sone M, Ikeya M, Watanabe A, Osafune K.

A Modular Differentiation System Maps Multiple Human Kidney Lineages from Pluripotent Stem Cells. Cell Rep. 2020 Apr 7;31(1):107476. doi: 10.1016/j.celrep.2020.03.040. PMID: 32268094

Mitsuzawa S, Ikeguchi R, Aoyama T, Ando M, Takeuchi H, Yurie H, Oda H, Noguchi T, Ohta S, Zhao C, Ikeya M, Matsuda S.

Induced pluripotent stem cell-derived mesenchymal stem cells prolong hind limb survival in a rat vascularized composite allotransplantation model. Microsurgery. 2019 Aug 31. doi: 10.1002/micr.30507. PMID: 31471984

Motoike S, Kajiya M, Komatsu N, Horikoshi S, Ogawa T, Sone H, Matsuda S, Ouhara K, Iwata T, Mizuno N, Fujita T, Ikeya M, Kurihara H.

Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis. Int J Mol Sci. 2019 Aug 15;20(16). pii: E3970. doi: 10.3390/ijms20163970. PMID: 31443173

Katsuno T, Belyantseva IA, Cartagena-Rivera AX, Ohta K, Crump SM, Petralia RS, Ono K, Tona R, Imtiaz A, Rehman A, Kiyonari H, Kaneko M, Wang YX, Abe T, Ikeya M, Fenollar-Ferrer C, Riordan GP, Wilson EA, Fitzgerald TS, Segawa K, Omori K, Ito J, Frolenkov GI, Friedman TB, Kitajiri SI.

TRIOBP-5 sculpts stereocilia rootlets and stiffens supporting cells enabling hearing. JCI Insight. 2019 June 20;4(12). pii: 128561. doi: 10.1172/jci.insight.128561. eCollection 2019 June 20. PMID: 31217345

Nakajima T, Ikeya M.

Insights into the biology of fibrodysplasia ossificans progressiva using patient-derived induced pluripotent stem cells. Regen Ther. 2019 May 10;11:25-30. doi: 10.1016/j.reth.2019.04.004. eCollection 2019 Dec. Review. PMID: 31193176

Kawai S, Yoshitomi H, Sunaga J, Alev C, Nagata S, Nishio M, Hada M, Koyama Y, Uemura M, Sekiguchi K, Maekawa H, Ikeya M, Tamaki S, Jin Y, Harada Y, Fukiage K, Adachi T, Matsuda S, Toguchida J.

In vitro bone-like nodules generated from patient-derived iPSCs recapitulate pathological bone phenotypes. Nat Biomed Eng. 2019 Jun 10. doi: 10.1038/s41551-019-0410-7. [Epub ahead of print] PMID: 31182836 [PubMed - as supplied by publisher]

Nakajima T*, Sakurai H, Ikeya M*.

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells. J Vis Exp. 2019 Apr 25; 146: e59359, doi:10.3791/59359

Iwashita M, Ohta H, Fujisawa T, Cho M, Ikeya M, Kidoaki S, Kosodo Y.

Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells. Sci Rep. 2019 Feb 28;9(1):3068. doi: 10.1038/s41598-018-38395-5. PMID: 30816128

Hino K, Zhao C, Horigome K, Nishio M, Okanishi Y, Nagata S, Komura S, Yamada Y, Toguchida J, Ohta A, Ikeya M*.

An mTOR Signaling Modulator Suppressed Heterotopic Ossification of Fibrodysplasia Ossificans Progressiva. Stem Cell Reports. 2018 Nov 1. pii: S2213-6711(18)30430-2. doi: 10.1016/j.stemcr.2018.10.007.

Nakajima T, Shibata M, Nishio M, Nagata S, Alev C, Sakurai H, Toguchida J, Ikeya M*

Modeling human somite development and fibrodysplasia ossificans progressiva with induced pluripotent stem cells Development. 2018 145: dev165431 doi: 10.1242/dev.165431 Published 23 August 2018.

Zhao C and Ikeya M.

Generation and Applications of Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells. Stem Cells Int. 2018 Jul 31; 2018:9601623. doi: 10.1155/2018/9601623. (Review)

Hino K, Horigome K, Nishio M, Komura S, Nagata S, Zhao C, Jin Y, Kawakami K, Yamada Y, Ohta A, Toguchida J*, Ikeya M*.

Activin-A enhances mTOR signaling to promote aberrant chondrogenesis in fibrodysplasia ossificans progressiva. J Clin Invest. 2017 Jul 31. pii: 93521. doi: 10.1172/JCI93521. [Epub ahead of print]

Chijimatsu R, Ikeya M, Yasui Y, Ikeda Y, Ebina K, Moriguchi Y, Shimamura K, Hart DA, Yoshikawa H and Nakamura N.

Characterization of Mesenchymal Stem Cell-Like Cells Derived From Human iPSCs via Neural Crest Development and Their Application for Osteochondral Repair. Stem Cells Int. 2017;2017:1960965. doi: 10.1155/2017/1960965. Epub 2017 May 10.

Horikiri T, Ohi H, Shibata M, Ikeya M, Ueno M, Sotozono C, Kinoshita S, Sato T.

SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research. PLoS One. 2017 Jan 20;12(1):e0170342.

Hayashi Y, Hsiao EC, Sami S, Lancero M, Schlieve CR, Nguyen T, Yano K, Nagahashi A, Ikeya M, Matsumoto Y, Nishimura K, Fukuda A, Hisatake K, Tomoda K, Asaka I, Toguchida J, Conklin BR, Yamanaka S.

BMP-SMAD-ID promotes reprogramming to pluripotency by inhibiting p16/INK4A-dependent senescence. Proc Natl Acad Sci U S A. 2016 Oct 28. pii: 201603668. [Epub ahead of print]

Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K.

Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Methods. 2015 Dec 18. [Epub ahead of print]

Hino K, Ikeya M*, Horigome K, Matsumoto Y, Ebise H, Nishio M, Sekiguchi K, Shibata M, Nagata S, Matsuda S, Toguchida J*.

Neofunction of ACVR1 in fibrodysplasia ossificans progressiva. Proc Natl Acad Sci U S A. 2015 Nov 30. [Epub ahead of print]

Tamaki S, Fukuta M, Sekiguchi K, Jin Y, Nagata S, Hayakawa K, Hineno S, Okamoto T, Watanabe M, Woltjen K, Ikeya M, Kato T Jr, Toguchida J.

SS18-SSX, the Oncogenic Fusion Protein in Synovial Sarcoma, Is a Cellular Context-Dependent Epigenetic Modifier. PLoS One. 2015 Nov 16;10(11):e0142991.

Jin Y, Elalaf H, Watanabe M, Tamaki S, Hineno S, Matsunaga K, Woltjen K, Kobayashi Y, Nagata S, Ikeya M, Kato T Jr, Okamoto T, Matsuda S, Toguchida J.

Mutant IDH1 Dysregulates the Differentiation of Mesenchymal Stem Cells in Association with Gene-Specific Histone Modifications to Cartilage- and Bone-Related Genes. PLoS One. 2015 Jul 10;10(7):e0131998.

Matsumoto Y†, Ikeya M†*, Hino K, Horigome K, Fukuta M, Watanabe M, Nagata S, Yamamoto T, Otsuka T, Toguchida J*.

New Protocol to Optimize iPS Cells for Genome Analysis of Fibrodysplasia Ossificans Progressiva. Stem Cells. 2015 Mar 13. Stem Cells. 2015 Jun;33(6):1730-42.

Yokoyama K, Ikeya M, Umeda K, Oda H, Nodomi S, Nasu A, Matsumoto Y, Izawa K, Horigome K, Kusaka T, Tanaka T, Saito MK, Yasumi T, Nishikomori R, Ohara O, Nakayama N, Nakahata T, Heike T, Toguchida J.

Enhanced chondrogenesis of iPS cells from neonatal-onset multisystem inflammatory disease occurs via the caspase-1-independent cAMP/PKA/CREB pathway. Arthritis Rheumatol. 2015 Jan;67(1):302-314.

Fukuta M, Nakai Y, Kirino K, Nakagawa M, Sekiguchi K, Nagata S, Matsumoto Y, Yamamoto T, Umeda K, Heike T, Okumura N, Koizumi N, Sato T, Nakahata T, Saito M, Otsuka T, Kinoshita S, Ueno M*, Ikeya M*, Toguchida J*.

Derivation of Mesenchymal Stromal Cells from Pluripotent Stem Cells through a Neural Crest Lineage using Small Molecule Compounds with Defined Media. PloS One. 2014 Dec 2;9(12):e112291

Matsumoto Y, Hayashi Y, Schlieve CR, Ikeya M*, Kim H, Nguyen TD, Sami S, Baba S, Barruet E, Nasu A, Asaka I, Otsuka T, Yamanaka S, Conklin BR, Toguchida J*, Hsiao EC*

Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation. Orphanet J Rare Dis. 2013 Dec 9;8(1):190. [Epub ahead of print]

Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H

Efficient and Reproducible Myogenic Differentiation from Human iPS Cells: Prospects for Modeling Miyoshi Myopathy In Vitro PLoS One. 2013;8(4)e61540

Hayakawa K, Ikeya M, Fukuta M, Woltjen K, Tamaki S, Takahara N, Kato T Jr, Sato S, Otsuka T, Toguchida J.

Identification of target genes of synovial sarcoma-associated fusion oncoprotein using human pluripotent stem cells. Biochem Biophys Res Commun. 2013 Mar 22;432(4):713-9.

Nasu A, Ikeya M, Yamamoto T, Watanabe A, Jin Y, Matsumoto Y, Hayakawa K, Amano N, Sato S, Osafune K, Aoyama T, Nakamura T, Kato T, Toguchida J.

Genetically matched human iPS cells reveal that propensity for cartilage and bone differentiation differs with clones, not cell type of origin. PLoS One. 2013;8(1):e53771. Epub 2013 Jan 31.

Ikeya M*, Fukushima K, Kawada M, Onishi S, Furuta Y, Yonemura S, Kitamura T, Nosaka T, Sasai Y.

Cv2, Functioning as a Pro-BMP Factor via Twisted Gastrulation, is Required for Early Development of Nephron Precursors. Dev. Biol. 337, 405-414 (2010)

Ikeya M*, Nosaka T, Fukushima K, Kawada M, Furuta Y, Kitamura T, Sasai Y.

Twisted gastrulation mutation suppresses skeletal defect phenotypes in Crossveinless 2 mutant mice. Mech. Dev. 125, 832-842 (2008)

Arakawa A, Matsuo-Takasaki M, Takai A, Inomata H, Matsumura M, Ikeya M, Takahashi K, Miyachi Y, Sasai N, Sasai Y.

The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo. Dev. Biol. 306, 160-169 (2007)

Ikeya M, Kawada M, Kiyonari H, Sasai N, Nakao K, Furuta Y, Sasai Y.

Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis. Development 133, 4463-4473 (2006)

Sakuragi M, Sasai N, Ikeya M, Kawada M, Onai T, Katahira T, Nakamura H, Sasai Y.

Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors. Mech. Dev. 123, 114-123 (2006)

Ikeya M*, Kawada M, Nakazawa Y, Sakuragi M, Sasai N, Ueno M, Kiyonari H, Nakao K, Sasai Y.

Gene disruption/knock-in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations. Int. J. Dev. Biol. 49, 807-823 (2005)

Mizuseki K, Sakamoto T, Watanabe K, Muguruma K, Ikeya M, Nishiyama A, Arakawa A, Suemori H, Nakatsuji N, Kawasaki H, Murakami F, Sasai Y.

Generation of neural crest-derived peripheral neurons and floor plate cells from mouse and primate embryonic stem cells. Proc. Natl. Acad. Sci. U S A. 100, 5828-5833 (2003)

Muroyama Y, Fujihara M, Ikeya M, Kondoh H, Takada S.

Wnt signaling plays an essential role in neuronal specification of the dorsal spinal cord. Genes Dev. 16, 548-553 (2002)

Nomi M, Oishi I, Kani S, Suzuki H, Matsuda T, Yoda A, Kitamura M, Itoh K, Takeuchi S, Takeda K, Akira S, Ikeya M, Takada S, Minami Y.

Loss of mRor1 enhances the heart and skeletal abnormalities in mRor2-deficient mice: redundant and pleiotropic functions of mRor1 and mRor2 receptor tyrosine kinases. Mol. Cell. Biol. 21, 8329-8335 (2001)

Kawauchi T, Ikeya M, Takada S, Ueda K, Shirai M, Takihara Y, Kioka N, Amachi T.

Expression of vinexin alpha in the dorsal half of the eye and in the cardiac outflow tract and atrioventricular canal. Mech. Dev. 106, 147-150 (2001)

Matsuda T, Nomi M, Ikeya M, Kani S, Oishi I, Terashima T, Takada S, Minami Y.

Expression of the receptor tyrosine kinase genes, Ror1 and Ror2, during mouse development. Mech. Dev. 105, 153-156 (2001)

Ikeya M, Takada S.

Wnt-3a is required for somite specification along the anteroposterior axis of the mouse embryo and for regulation of cdx-1 expression. Mech. Dev. 103, 27-33 (2001)

Takeuchi S, Takeda K, Oishi I, Nomi M, Ikeya M, Itoh K, Tamura S, Ueda T, Hatta T, Otani H, Terashima T, Takada S, Yamamura H, Akira S, Minami Y.

Mouse Ror2 receptor tyrosine kinase is required for the heart development and limb formation. Genes Cells. 5, 71-78 (2000)

Ikeya M, Takada S.

Wnt signaling from the dorsal neural tube is required for the formation of the medial dermomyotome. Development 125, 4969-4976 (1998)

Ikeya M, Lee SM, Johnson JE, McMahon AP, Takada S.

Wnt signalling required for expansion of neural crest and CNS progenitors. Nature 389, 966-970 (1997)
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