Research Activities

Research Activities

Home › Research Activities › Principal Investigators › Noriyuki Tsumaki (Professor)

Principal Investigators

Dept. of Clinical Application 
Noriyuki Tsumaki (Professor)

Publications

Chen X, Yamashita A, Morioka M, Senba T, Kamatani T, Watanabe A, Kosai A, Tsumaki N

Integration capacity of human iPS cell-derived cartilage Tissue Eng Part A. 21 Aug 2018 doi: 10.1089/ten.TEA.2018.0133

Kimura T, Yamashita A, Ozono K, Tsumaki N.

Limited Immunogenicity of Human Induced Pluripotent Stem Cell-Derived Cartilages. Tissue Eng Part A. 2016 Dec;22(23-24):1367-1375. Epub 2016 Oct 31.

Yasuhito Yahara, Hiroshi Takemori, Minoru Okada, Azuma Kosai, Akihiro Yamashita, Tomohito Kobayashi, Kaori Fujita, Yumi Itoh, Masahiro Nakamura, Hiroyuki Fuchino, Nobuo Kawahara, Naoshi Fukui, Akira Watanabe, Tomoatsu Kimura and Noriyuki Tsumaki

Pterosin B prevents chondrocyte hypertrophy and osteoarthritis in mice by inhibiting Sik3 Nature Communications, 2016; 7:10959

Yamashita, A., Morioka, M., Yahara, Y., Okada, M., Kobayashi, T., Kuriyama, S., Matsuda, S., and Tsumaki, N.

Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs. Stem cell reports, 2015; 4: 404-418.

Okada M, Ikegawa S, Morioka M, Yamashita A, Saito A, Sawai H, Murotsuki J, Ohashi H, Okamoto T, Nishimura G, Imaizumi K, Tsumaki N.

Modeling type II collagenopathy skeletal dysplasia by directed conversion and induced pluripotent stem cells. Hum Mol Genet, 2015; 24: 299-313.

Yamashita A, Morioka M, Kishi H, Kimura T, Yahara Y, Okada M, Fujita K, Sawai H, Ikegawa S, Tsumaki N.

Statin treatment rescues FGFR3 skeletal dysplasia phenotypes. Nature, 2014; 513: 507-511.

Tam WL, Dorien FO, Hiramatsu K, Tsumaki N, Luyten FP, Roberts SJ.

Sox9 reprogrammed dermal fibroblasts undergo hypertrophic differentiation in vitro and trigger endochondral ossification in vivo. Cell Reprogram, 2014; 16: 29-39.

Seki S, Tsumaki N, Motomura H, Nogami M, Kawaguchi Y, Hori T, Suzuki K, Yahara Y, Higashimoto M, Oya T, Ikegawa S, Kimura T.

Cartilage intermediate layer protein promotes lumbar disc degeneration. Biochem Biophys Res Commun, 2014; 446: 876-881.

Minegishi Y, Sakai Y, Yahara Y, Akiyama H, Yoshikawa H, Hosokawa K, Tsumaki N.

Cyp26b1 within the growth plate regulates bone growth in juvenile mice. Biochem Biophys Res Commun, 2014; 454: 12-18.

Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N.

Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors. PLoS One, 2013; 8: e77365.

Minegishi Y, Hosokawa K, Tsumaki N.

Time-lapse observation of the dedifferentiation process in mouse chondrocytes using chondrocyte-specific reporters. Osteoarthritis Cartilage, 2013; 21: 1968-1975.

Uebi T, Itoh Y, Hatano O, Kumagai A, Sanosaka M, Sasaki T, Sasagawa S, Doi J, Tatsumi K, Mitamura K, Morii E, Aozasa K, Kawamura T, Okumura M, Nakae J, Takikawa H, Fukusato T, Koura M, Nish M, Hamsten A, Silveira A, Bertorello AM, Kitagawa K, Nagaoka Y, Kawahara H, Tomonaga T, Naka T, Ikegawa S, Tsumaki N, Matsuda J, Takemori H.

Involvement of SIK3 in glucose and lipid homeostasis in mice. PLoS One, 2012; 7: e37803.

Sasagawa S, Takemori H, Uebi T, Ikegami D, Hiramatsu K, Ikegawa S, Yoshikawa H, Tsumaki N.

SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice. Development, 2012; 139: 1153-1163.

Nishimura R, Wakabayashi M, Hata K, Matsubara T, Honma S, Wakisaka S, Kiyonari H, Shioi G, Yamaguchi A, Tsumaki N, Akiyama H, Yoneda T.

Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J Biol Chem, 2012; 287: 33179-33190.

Miura K, Namba N, Fujiwara M, Ohata Y, Ishida H, Kitaoka T, Kubota T, Hirai H, Higuchi C, Tsumaki N, Yoshikawa H, Sakai N, Michigami T, Ozono K.

An Overgrowth Disorder Associated with Excessive Production of cGMP Due to a Gain-of-Function Mutation of the Natriuretic Peptide Receptor 2 Gene. PLoS One, 2012; 7: e42180.

Hiramatsu, K., Sasagawa, S., Outani, H., Nakagawa, K., Yoshikawa, H. and Tsumaki, N.

Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors. J. Clin. Invest., 121: 640-57, 2011.

Ikegami, D., Akiyama, H., Suzuki, A., Nakamura, T., Nakano, T., Yoshikawa, H. and Tsumaki, N

Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways. Development, 138: 1507-19, 2011.

Okamoto, M., Murai, J., Imai, Y., Ikegami, D., Kamiya, N., Kato, S., Mishina, Y., Yoshikawa, H. and Tsumaki, N

Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice. J. Bone Miner. Res., 26: 2511-22, 2011.

Ikegami, D., Iwai, T., Ryo, S., Gu, N., Sugiyama, T., Oh, I., Yoshikawa, H. and Tsumaki, N

Identification of small molecular compounds and fabrication of its aqueous solution by laser-ablation, expanding primordial cartilage Osteoarthritis Cartilage, 19: 233-41, 2011.

Outani, H., Okada, M., Hiramatsu, K., Yoshikawa, H. and Tsumaki, N

Induction of chondrogenic cells from dermal fibroblast culture by defined factors does not involve a pluripotent state. Biochem. Biophys. Res. Commun., 411: 607-12, 2011.

Hiramatsu, K., Iwai, T., Yoshikawa, H. and Tsumaki, N

Expression of dominant negative TGF-beta receptors inhibits cartilage formation in conditional transgenic mice. J. Bone Miner. Metab., 29: 493-500, 2011.

Nakamura, Y., Yamamoto, K., He, X., Otsuki, B., Kim, Y., Murao, H., Soeda, T., Tsumaki, N., Deng, J. M., Zhang, Z., Behringer, R. R., Crombrugghe, B., Postlethwait, J. H., Warman, M. L., Nakamura, T. and Akiyama, H

Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25 Nat Commun, 2: 251, 2011.

Suzuki, D., Yamada, A., Amano, T., Yasuhara, R., Kimura, A., Sakahara, M., Tsumaki, N., Takeda, S., Tamura, M., Nakamura, M., Wada, N., Nohno, T., Shiroishi, T., Aiba, A. and Kamijo, R

Essential mesenchymal role of small GTPase Rac1 in interdigital programmed cell death during limb development. Dev. Biol., 335: 396-406, 2009.

Iwai, T., Murai, J., Yoshikawa, H. and Tsumaki, N.

Smad7 Inhibits chondrocyte differentiation at multiple steps during endochondral bone formation and down-regulates p38 MAPK pathways J. Biol. Chem., 283: 27154-64, 2008.

Murai, J., Ikegami, D., Okamoto, M., Yoshikawa, H. and Tsumaki, N

Insulation of the ubiquitous Rxrb promoter from the cartilage-specific adjacent gene, Col11a2 J. Biol. Chem., 283: 27677-87, 2008.

Nampei, A., Hashimoto, J., Koyanagi, J., Ono, T., Hashimoto, H., Tsumaki, N., Tomita, T., Sugamoto, K., Nishimoto, N., Ochi, T. and Yoshikawa, H.:

Characteristics of fracture and related factors in patients with rheumatoid arthritis. Modern rheumatology / the Japan Rheumatism Association, 18: 170-6, 2008.

Sugiki, T., Uyama, T., Toyoda, M., Morioka, H., Kume, S., Miyado, K., Matsumoto, K., Saito, H., Tsumaki, N., Takahashi, Y., Toyama, Y. and Umezawa, A.

Hyaline cartilage formation and enchondral ossification modeled with KUM5 and OP9 chondroblasts. J. Cell. Biochem., 100: 1240-54, 2007.

Iwai, T., Harada, Y., Imura, K., Iwabuchi, S., Murai, J., Hiramatsu, K., Myoui, A., Yoshikawa, H. and Tsumaki, N.

Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite ceramic J. Bone Miner. Metab., 25: 392-9, 2007.

Okamoto, M., Murai, J., Yoshikawa, H. and Tsumaki, N

Bone morphogenetic proteins in bone stimulate osteoclasts and osteoblasts during bone development. J. Bone Miner. Res., 21: 1022-33, 2006.

Hirao, M., Tamai, N., Tsumaki, N., Yoshikawa, H. and Myoui, A

Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification. J. Biol. Chem., 281: 31079-92, 2006.

Tsumaki, N., Kakiuchi, M., Sasaki, J., Ochi, T. and Yoshikawa, H

Low-intensity pulsed ultrasound accelerates maturation of callus in patients treated with opening-wedge high tibial osteotomy by hemicallotasis J. Bone Joint Surg. Am., 86-A: 2399-405, 2004.

Horiki, M., Imamura, T., Okamoto, M., Hayashi, M., Murai, J., Myoui, A., Ochi, T., Miyazono, K., Yoshikawa, H. and Tsumaki, N

Smad6/Smurf1 overexpression in cartilage delays chondrocyte hypertrophy and causes dwarfism with osteopenia. J. Cell Biol., 165: 433-45, 2004.

Katayama, R., Wakitani, S., Tsumaki, N., Morita, Y., Matsushita, I., Gejo, R. and Kimura, T

Repair of articular cartilage defects in rabbits using CDMP1 gene-transfected autologous mesenchymal cells derived from bone marrow Rheumatology (Oxford). 43: 980-5, 2004.

Matsui, Y., Chansky, H. A., Barahmand-Pour, F., Zielinska-Kwiatkowska, A., Tsumaki, N., Myoui, A., Yoshikawa, H., Yang, L. and Eyre, D. R

COL11A2 collagen gene transcription is differentially regulated by EWS/ERG sarcoma fusion protein and wild-type ERG J. Biol. Chem., 278: 11369-75, 2003

Tsumaki, N., Nakase, T., Miyaji, T., Kakiuchi, M., Kimura, T., Ochi, T. and Yoshikawa, H

Bone morphogenetic protein signals are required for cartilage formation and differently regulate joint development during skeletogenesis J. Bone Miner. Res., 17: 898-906, 2002.

Nakase, T., Sugamoto, K., Miyamoto, T., Tsumaki, N., Luyten, F. P., Inui, H., Myoui, A., Tomita, T. and Yoshikawa, H

Activation of cartilage-derived morphogenetic protein-1 in torn rotator cuff. Clin Orthop Relat Res: 140-5, 2002

Matsui, Y., Araki, N., Tsuboi, H., Tsumaki, N., Nakata, K., Kawabata, H., Eyre, D. R. and Yoshikawa, H

Differential expression of aggrecan mRNA isoforms by chondrosarcoma cells Clin Orthop Relat Res: 140-5, 2002

Nakase, T., Ariga, K., Yonenobu, K., Tsumaki, N., Luyten, F. P., Mukai, Y., Sato, I. and Yoshikawa, H

Activation and localization of cartilage-derived morphogenetic protein-1 at the site of ossification of the ligamentum flavum Eur. Spine J., 10: 289-94, 2001.

Matsui, Y., Nakata, K., Araki, N., Ozono, K., Fujita, Y., Tsumaki, N., Kawabata, H., Yasui, N., Sekiguchi, K. and Yoshikawa, H

Alternative splicing of fibronectin transcripts in osteochondrogenic tumors Anticancer Res., 21: 1103-6, 2001

Matsui, Y., Yasui, N., Kawabata, H., Ozono, K., Nakata, K., Mizushima, T., Tsumaki, N., Kataoka, E., Fujita, Y. and Ochi, T

A novel type X collagen gene mutation (G595R) associated with Schmid-type metaphyseal chondrodysplasia. J. Hum. Genet., 45: 105-8, 2000.

Liu, Y., Li, H., Tanaka, K., Tsumaki, N. and Yamada, Y.

Identification of an enhancer sequence within the first intron required for cartilage-specific transcription of the alpha2(XI) collagen gene J. Biol. Chem., 275: 12712-8, 2000.

Tsumaki, N., Tanaka, K., Arikawa-Hirasawa, E., Nakase, T., Kimura, T., Thomas, J. T., Ochi, T., Luyten, F. P. and Yamada, Y.

Role of CDMP-1 in skeletal morphogenesis: promotion of mesenchymal cell recruitment and chondrocyte differentiation. J. Cell Biol., 144: 161-73, 1999

Tsumaki, N., Kimura, T., Tanaka, K., Kimura, J. H., Ochi, T. and Yamada, Y.

Modular arrangement of cartilage- and neural tissue-specific cis-elements in the mouse alpha2(XI) collagen promoter. J. Biol. Chem., 273: 22861-4, 1998.

Sugimoto, M., Kimura, T., Tsumaki, N., Matsui, Y., Nakata, K., Kawahata, H., Yasui, N., Kitamura, Y., Nomura, S. and Ochi, T

Differential in situ expression of alpha2(XI) collagen mRNA isoforms in the developing mouse Cell Tissue Res., 292: 325-32, 1998.

Matsui, Y., Yasui, N., Kimura, T., Tsumaki, N., Kawabata, H. and Ochi, T.

Genotype phenotype correlation in achondroplasia and hypochondroplasia. J. Bone Joint Surg. Br., 80: 1052-6, 1998.

Matsui, Y., Kimura, T., Tsumaki, N., Nakata, K., Yasui, N., Araki, N., Hashimoto, N., Uchida, A. and Ochi, T

Splicing patterns of type XI collagen transcripts act as molecular markers for osteochondrogenic tumors Cancer Lett., 124: 143-8, 1998.

Tsumaki, N., Kimura, T., Matsui, Y., Nakata, K. and Ochi, T.

Separable cis-regulatory elements that contribute to tissue- and site-specific alpha 2(XI) collagen gene expression in the embryonic mouse cartilage. J. Cell Biol., 134: 1573-82, 1996.

Tsumaki, N., Sugimoto, M., Nakata, K., Matsui, Y., Ochi, T. and Kimura, T.

Separable regulatory elements from pro-alpha 2 (XI) collagen gene drive distinct patterns of cartilage-specific expression in transgenic mice. Ann. N. Y. Acad. Sci., 785: 340-2, 1996.

Matsui, Y., Kimura, T., Tsumaki, N., Yasui, N. and Ochi, T.

A recurrent 1992delCT mutation of the type X collagen gene in a Japanese patient with Schmid metaphyseal chondrodysplasia. Jpn. J. Hum. Genet., 41: 339-42, 1996.

Kimura, T., Nakata, K., Tsumaki, N., Miyamoto, S., Matsui, Y., Ebara, S. and Ochi, T.

Progressive degeneration of articular cartilage and intervertebral discs. An experimental study in transgenic mice bearing a type IX collagen mutation Int. Orthop., 20: 177-81, 1996

Iwasaki, M., Okada, K., Tsumaki, N., Obata, H., Shirasaki, N. and Oka, S

Cervical spondylotic radiculopathy involving two adjacent nerve roots. Anterior decompression through a single level intervertebral approach. Int. Orthop., 20: 137-41, 1996.

Tsumaki, N. and Kimura, T.

Differential expression of an acidic domain in the amino-terminal propeptide of mouse pro-alpha 2(XI) collagen by complex alternative splicing. J. Biol. Chem., 270: 2372-8, 1995.
Books

Tsumaki N.

Chapter 6 Cartilage Regeneration Using Induced Pluripotent Stem Cell Technologies, In A Tissue Regeneration Approach to Bone and Cartilage Repair, 2015, Hala Zreiqat, Vicki Rosen, Colin Dunstan (eds.) Springer International Publishing Switzerland

Yoshikawa H, Tsumaki N, Myoui A

Chapter 14 Bone Biology: Development and Regeneration Mechanisms, in Physiological and Pathological Conditions, In Strategies in Regenerative Medicine, 2009, Matteo Santin editor, Springer, ISBN 978-0-387-74659-3

Tsumaki N, Liu Y, Yamada Y, and Krebsbach P

Enhancer analysis of the a1(II) and a2(XI) collagen genes in transfected chondrocytes and transgenic mice. In Streuli, C. H., and Grant, M. E. (eds.) Extracellular Matrix Protocols, Methods Mol Biol, 139, Humana press, Totowa, NJ, USA, pp. 187-195, 2000.

Kimura T, Nakata K, Tsumaki N, Ono K

Altered chondrocyte gene expression in articular cartilage matrix components and susceptibility to cartilage destruction. In Mow, V.C. (Ed.) Cell Mechanics and Cellular Engineering, Springer-Verlag, New York, USA, pp. 445-456, 1994.
Review

Tsumaki N, Okada M, Yamashita A.

iPS cell technologies and cartilage regeneration. Bone 2015; 70: 48-54.

Karagiannis P, Tsumaki N.

Cell reprogramming for skeletal dysplasia drug repositioning. Cell Cycle 2014; 13: 3791-3792.

Tsumaki, N.*, and Yoshikawa, H

The role of bone morphogenetic proteins in endochondral bone formation. Cytokine Growth Factor Rev 16:279-285, 2005.
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