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研究活動
Research Activities

Home › 研究活動 › 主任研究者 › 江藤 浩之 教授

主任研究者
Principal Investigator

臨床応用研究部門 江藤 浩之 教授

発表論文

Yukitaka Ito, Sou Nakamura, Naoshi Sugimoto, Tomohiro Shigemori, Yoshikazu Kato, Mikiko Ohno, Shinya Sakuma, Keitaro Ito, Hiroki Kumon, Hidenori Hirose, Haruki Okamoto, Masayuki Nogawa, Mio Iwasaki, Shunsuke Kihara, Kosuke Fujio, Takuya Matsumoto, Natsumi Higashi, Kazuya Hashimoto, Akira Sawaguchi, Ken-ichi Harimoto, Masato Nakagawa, Takuya Yamamoto, Makoto Handa, Naohide Watanabe, Eiichiro Nishi, Fumihito Arai, Satoshi Nishimura, Koji Eto.

Turbulence activates platelet biogenesis to enable clinical scale ex vivo production Cell. 2018 Jul 26;174(3):636-648.e18. doi: 10.1016/j.cell.2018.06.011. Epub 2018 Jul 12.

Sugimoto N, Eto K.

Platelet production from induced pluripotent stem cells. J Thromb Haemost. 2017 Sep;15(9):1717-1727. doi: 10.1111/jth.13736. Epub 2017 Jul 28.

Watanabe N, Nogawa M, Ishiguro M, Maruyama H, Shiba M, Satake M, Eto K, Handa M.

Refined methods to evaluate the in vivo hemostatic function and viability of transfused human platelets in rabbit models. Transfusion. 2017 Aug;57(8):2035-2044. doi: 10.1111/trf.14189. Epub 2017 Jul 5.

Aihara A, Koike T, Abe N, Nakamura S, Sawaguchi A, Nakamura T, Sugimoto N, Nakauchi H, Nishino T, Eto K*.

Novel TPO receptor agonistA-316 contributes to platelet biogenesis from human iPS cells. Blood Advances. 1(7):468-476, 2017

Hirata S, Murata T, Suzuki D, Nakamura S, Jono-Ohnishi R, Hirose H, Sawaguchi A, , Nishimura S, Sugimoto N, Eto K*.

Selective inhibition of ADAM17 efficiently mediates glycoprotein Iba retention during ex vivo generation of human induced pluripotent stem cell-derived platelets. Stem Cells Transl Med. 6(3):720-730, 2017.

Peter Karagiannias, Hiroshi Endo, Koji Eto.

Generating Blood from iPS Cells. Molecular and Cellular Biology of Platelet Formation: Implications in Health and Disease. (Book) pp 399-420, 2017

Hirata S, Murata T, Suzuki D, Nakamura S, Jono-Ohnishi R, Hirose H, Sawaguchi A, Nishimura S, Sugimoto N, Eto K.

Selective Inhibition of ADAM17 Efficiently Mediates Glycoprotein Ibα Retention During Ex Vivo Generation of Human Induced Pluripotent Stem Cell-Derived Platelets. Stem Cells Transl Med. 2017 Mar;6(3):720-730. doi: 10.5966/sctm.2016-0104. Epub 2016 Oct 5.

Eto K*, Kunishima S.

Linkage between the mechanisms of thrombocytopenia and thrombosis. Blood. 2016 Mar 10;127(10):1234-1241. doi: 10.1182/blood-2015-07-607903. Epub 2016 Jan 19. Review.

Fujii T, Sakata A, Nishimura S, Eto K, Nagata S.

TMEM16F is required for phosphatidylserine exposure and microparticle release in activated mouse platelets. PNAS. 2015 Oct 13;112(41):12800-5. doi: 10.1073/pnas.1516594112. Epub 2015 Sep 28.2

Karagiannis P, Eto K*.

Manipulating megakaryocytes to manufacture platelets ex vivo. J Thromb Haemost. 2015 Jun 13 ; Suppl 1:S47-53. doi: 10.1111/jth.12946

Nishimura S, Nagasaki M, Kunishima S, Sawaguchi A, Sakata A, Sakaguchi H, Ohmori T, Manabe I, Italiano JE Jr, Ryu T, Takayama N, Komuro I, Kadowaki T, Eto K*, Nagai R.

IL-1α induces thrombopoiesis through megakaryocyte rupture in response to acute platelet needs. J Cell Biol. 2015 May 11;209(3):453-66. doi: 10.1083/jcb.201410052

Eto K*.

Platelet biogenesis wears silkworm cocoons. Blood. 2015 April 2 ; 125 (14)2181-83.

Toriumi T, Takayama N, Murakami M, Sato M, Yuguchi M, Yamazaki Y, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda MJ.

Characterization of mesenchymal progenitor cells in the crown and root pulp of primary teeth. Biomed Res. 2015;36(1):31-45. doi: 10.2220/biomedres.36.31.

Nishimura S, Nagasaki M, Okudaira S, Aoki J, Ohmori 4, Ohkawa R, Nakamura K, Igarashi K, Yamashita H, Eto K, Uno K, Hayashi N, Kadowaki T, Komuro I, Yatomi Y, Nagai R.

ENPP2 contributes to adipose tissue expansion and insulin resistance in Diet-Induced obesity. Diabetes. 2014 Dec; 63(12):4154-64.

Ishii T, Eto K.

Fetal stem cell transplantation: Past, present, and future. World J Stem Cells. 2014 Sept. 26; 6(4): 404-420.

Ochi K, Takayama N, Hirose S, Nakahata T, Nakauchi H, Eto K. *

Multicolor staining of globin subtypes reveals impaired globin switching during erythropoiesis in human pluripotent stem cells. Stem Cells Transl Med. 2014 Jul;3(7):792-800.

Hirata N, Nakagawa M, Fujibayashi Y, Yamauchi K, Murata A, Minami I, Tomioka M, Kondo T, Kuo TF, Endo H, Inoue H, Sato S, Ando S, Kawazoe Y, Aiba K, Nagata K, Kawase E, Chang YT, Suemori H, Eto K, Nakauchi H, Yamanaka S, Nakatsuji N, Ueda K, Uesugi M.

A Chemical Probe that Labels Human Pluripotent Stem Cells. Cell Rep. 2014 Mar 27;6(6):1165-74.

Nakamura S, Takayama N, Hirata S, Seo H, Endo H, Ochi K, Fujita KI, Koike T, Harimoto KI, Watanabe A, Okita K, Takahashi N, Sawaguchi A, Yamanaka S, Nakauchi H, Nishimura S, Eto K.

Expandable megakaryocyte cell lines enable clinically-aplicable generation of platelets from human induced pluripotent stem cells. Cell Stem Cell. 2014 Feb 12;14(4):535-548. doi: 10.1016/j.stem.2014.01

Hirose S, Takayama N, Nakamura S, Nagasawa K, Ochi K, Hirata S, Yamazaki S, Yamaguchi T, Otsu M, Sano S, Takahashi N, Sawaguchi A, Ito M, Kato T, Nakauchi H, Eto K.

Immortalization of erythroblasts by c-MYC and BCL-XL enables large-scale erythrocyte production from human pluripotent stem cells. Stem Cell Reports. 2013 Dec 5;1(6):499-508. doi: 10.1016/j.stemcr.2013.10.010. eCollection 2013.

Nishimura S, Manabe I, Takaki S, Nagasaki M, Otsu M, Yamashita H, Sugita J, Yoshimura K, Eto K, Komuro I, Kadowaki T, Nagai R.

Adipose natural regulatory B cells negatively control adipose tissue inflammation. Cell Metab. 2013 Oct 22. pii: S1550-4131(13)00386-0. doi: 10.1016/j.cmet.2013.09.017. [Epub ahead of print]

Hirata S, Takayama N, Jono-Ohnishi R, Endo H, Nakamura S, Dohda T, Nishi M, Hamazaki Y, Ishii EI, Kaneko S, Otsu M, Nakauchi H, Kunishima S, Eto K.

Congenital amegakaryocytic thrombocytopenia iPS cells exhibit defective MPL-mediated signaling. J Clin Invest. 2013 Sep 3;123(9):3802-14. doi: 10.1172/JCI64721. Epub 2013 Aug 1.

Nakagawa Y, Nakamura S, Nakajima M, Endo H, Dohda T, Takayama N, Nakauchi H, Arai F, Fukuda T, Eto K.

Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes. Exp Hematol. 2013 Aug;41(8):742-8. doi: 10.1016/j.exphem.2013.04.007. Epub 2013 Apr 22

Oda A, Eto K.

WASPs and WAVEs: from molecular function to physiology in hematopoietic cells. Semin Cell Dev Biol. 2013 Apr;24(4):308-13. doi: 10.1016/j.semcdb.2013.03.002. Epub 2013 Mar 14

Nishimura T, Kaneko S, Kawana-Tachikawa A, Tajima Y, Goto H, Zhu D, Nakayama-Hosoya K, Iriguchi S, Uemura Y, Shimizu T, Takayama N, Yamada D, Nishimura K, Ohtaka M, Watanabe N, Takahashi S, Iwamoto A, Koseki H, Nakanishi M, Eto K, Nakauchi H.

Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation. Cell Stem Cell. 2013 Jan 3;12(1):114-26. doi: 10.1016/j.stem.2012.11.002.

Kumano K, Arai S, Hosoi M, Taoka K, Takayama N, Otsu M, Nagae G, Ueda K, Nakazaki K, Kamikubo Y, Eto K, Aburatani H, Nakauchi H, Kurokawa M.

Generation of induced pluripotent stem cells from primary chronic myelogenous leukemia patient samples. Blood. 2012 Jun 28;119(26):6234-42.

Takayama N, Eto K.

Pluripotent stem cells reveal the developmental biology of humanmegakaryocytes and provide a source of platelets for clinical application. Cell Mol Life Sci. 2012 Apr 24. [Epub ahead of print] in press

Takayama N, Eto K.

In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells. Methods Mol Biol. 2012;788:205-17.

Nishimura S, Manabe I, Nagasaki M, Kakuta S, Iwakura Y, Takayama N, Ooehara J, Otsu M, Kamiya A, Petrich BG, Urano T, Kadono T, Sato S, Aiba A,Yamashita H, Sugiura S, Kadowaki T, Nakauchi H, Eto K*, Nagai R.

In vivo imaging visualizes discoid platelet aggregations without endothelium disruption andimplicates contribution of inflammatory cytokine and integrin signaling. Blood. 2012 Feb 23;119(8):e45-56. * corresponding author

Umemoto T, Yamato M, Ishihara J, Shiratsuchi Y, Utsumi M, Morita Y, Tsukui H, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich BG, Nakauchi H, Eto K*, Okano T.

Integrin-avb3 regulates thrombopoietin-mediated maintenance ofhematopoietic stem cells. Blood. 2012 Jan 5;119(1):83-94. * corresponding author

Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H.

Heterozygous ITGA2B R995W mutation inducing constitutive activation of the aIIbβ3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia Blood 117, 5479-5484 (2011).

Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M.

Reduction ofN-glycolylneuraminic acid in human induced pluripotent stem cells generated or cultured under feeder- and serum-free defined conditions. PLoS One. 2010 Nov 23;5(11):e14099.

Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K.

Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells J Exp Med 207, 2817-30 (2010).

Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K.

Lnk regulates integrin alphaIIbbeta3 outside-in signaling in mouse platelets, leading to stabilization of thrombus development in vivo. J Clin Invest 120, 179-90 (2010).

Oki T, Eto K, Izawa K, Yamanishi Y, Inagaki N, Frampton J, Kitamura T, Kitaura J.

Evidence that integrin aIIbβ3-dependent interaction of mast cells with fibrinogen exacerbates chronic inflammation. J Biol Chem 284, 31463-72 (2009).

Okabe M, Otsu M, Ahn DH, Kobayashi T, Wakiyama Y, Morita Y, Onodera M, Eto K, Ema H, Nakauchi H.

Definitive proof for direct reprogramming of hematopoietic cells to pluripotency. Blood 114, 1764-7 (2009).

Nishimura S, Manabe I, Nagasaki M, Eto K, Yamashita H, Ohsugi M, Otsu M, Hara K, Ueki K, Sugiura S, Yoshimura K, Kadowaki T, Nagai R.

CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity. Nat Med 15, 914-20 (2009).

Ogaeri T, Eto K, Otsu M, Ema H, Nakauchi H.

The actin polymerization regulator WAVE2 is required for early bone marrow repopulation by hematopoietic stem cells. Stem Cells 27, 1120-9 (2009).

Yamazaki S, Iwama A, Takayanagi S, Eto K, Ema H, Nakauchi H.

TGF-β induces hibernation of hematopoietic stem cells in the bone marrow niche. Blood 113, 1250-6 (2009).

Nishikii H, Eto K*, Tamura N, Hattori K, Heissig B, Kanaji T, Sawaguchi A, Goto S, Ware J, Nakauchi H.

Metalloproteinase regulation improves in vitro generation of efficacious platelets from mouse embryonic stem cells. J Exp Med 205, 1917-27 (2008). *corresponding

Takayama N, Nishikii H, Usui J, Tsukui H, Sawaguchi A, Hiroyama T, Eto K*, Nakauchi H.

Generation of functional platelets from human embryonic stem cells in vitro via ES-sacs, VEGF-promoted structures that concentrate hematopoietic progenitors. Blood 111, 5298-5306 (2008). *corresponding

Takizawa H, Eto K, Yoshikawa A, Nakauchi H, Takatsu K, Takaki S.

Growth and maturation of megakaryocytes is regulated by Lnk/SH2B3 adaptor protein through crosstalk between cytokine- and integrin-mediated signals Exp Hematol 36, 897-906 (2008).

Eto K*, Nishikii H, Ogaeri T, Suetsugu S, Kamiya A, Kobayashi T, Yamazaki D, Oda A, Takenawa T, Nakauchi H.

The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading: implications for platelet biogenesis and spreading machinery. Blood 110, 3637-3647 (2007). *corresponding

Yamazaki S, Iwama A, Takayanagi SI, Morita Y, Eto K, Ema H, Nakauchi H.

Cytokine signals modulated via lipid rafts mimic niche signals and induce hibernation in hematopoietic stem cells. EMBO J 25, 3515-3523 (2006).

Matsubara A, Iwama A, Yamazaki S, Furuta C, Hirasawa R, Morita Y, Osawa M, Motohashi T, Eto K, Ema H, Kitamura T, Vestwever D, Nakauchi H.

Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development. J Exp Med 202, 1483-1492 (2005).

Kitaura J, Eto K, Kinoshita T, Kawakami Y, Lowell CA, Kawakami T.

Regulation of highly cytokinergic IgE-induced mast cell adhesion by Src, Syk, Tec, and PKC family kinases. . J Immunol 174, 4495-4504 (2005).

Tadokoro S, Shattil SJ, Eto K, Tai V, Liddington RC, de Pereda JM, Ginsberg MH, Calderwood DA.

Talin binding to integrin beta tails: a final common step in integrin activation. Science 302, 103-106 (2003).

Eto K, Murphy R, Kerrigan SW, Bertoni A, Stuhlmann H, Nakano T, Leavitt AD, Shattil SJ.

Development and analysis of megakaryocytes from murine embryonic stem cells. Differentiation of Embryonic Stem Cells (Book) 365, 142-158 (2003).

Eto K, Murphy R, Kerrigan SW, Bertoni A, Stuhlmann H, Nakano T, Leavitt AD, Shattil SJ.

Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling. Proc. Natl. Acad. Sci. U S A. 99, 12819-12824 (2002).

Sakakibara M, Goto S, Eto K, Tamura N, Isshiki T, Handa S.

Application of ex vivo flow chamber system for assessment of stent thrombosis. Arterioscler Thromb Vasc Biol 22, 1360-1364 (2002).

Goto S, Tamura N, Eto K, Ikeda Y, Handa S.

Functional significance of adenosine 5'-diphosphate receptor (P2Y12) in platelet activation initiated by binding of von Willebrand factor to platelet GPIb-alpha induced by conditions of high shear rate. Circulation 105, 2531-2536 (2002).

Bertoni A, Tadokoro S, Eto K, Pampori N, Parise LV, White GC, Shattil SJ.

Relationships between rap1b, affinity modulation of integrin aIIbβ3, and the actin cytoskeleton. J Biol Chem 277, 25715-25721 (2002).

Obergfell A, Eto K, Mocsai A, Buensuceso C, Moores SL, Brugge JS, Lowell CA, Shattil SJ.

Coordinate interactions of Csk, Src, and Syk kinases with aIIbβ3 initiate integrin signaling to the cytoskeleton. J Cell Biol 157, 265-275 (2002).

Eto K, Huet C, Tarui T, Kupriyanov S, Liu HZ, Puzon-McLaughlin W, Zhang XP, Sheppard D, Engvall E, Takada Y.

Functional classification of ADAMs based on a conserved motif for binding to integrin a9β1; implications for sperm-egg binding and other cell interactions. J Biol Chem 277, 17804-17810 (2002).

Eto K, Puzon-McLaughlin W, Sheppard D, Sehara-Fujisawa A, Zhang XP, Takada Y.

RGD-independent binding of integrin α9β1 to the ADAM-12 and - 15 disintegrin domains mediates cell-cell interaction. J Biol Chem 275, 34922-34930 (2000).

Goto S, Eto K, Ikeda Y, Handa S.

Abciximab not RGD peptide inhibits von Willebrand factor-dependent platelet activation under shear. Lancet 353, 809 (1999).

Eto K, Isshiki T, Yamamoto H, Takeshita S, Ochiai M, Yokoyama N, Yoshimoto R, Ikeda Y, Sato T.

AJvW-2, an anti-vWF monoclonal antibody, inhibits enhanced platelet aggregation induced by high shear stress in platelet rich plasma from patients with acute coronary syndromes. Arterioscler Thromb Vasc Biol 19, 877-882 (1999).

Takeshita S, Isshiki T, Ochiai M, Eto K, Mori H, Tanaka E, Umetani K, Sato T.

Endothelium-dependent relaxation of collateral microvessels after intramuscular gene transfer of vascular endothelial growth factor in a rat model of hindlimb ischemia. Circulation 98, 1261-1263 (1998).

Ochiai M, Isshiki T, Eto K, Yokoyama N, Fusano T, Takeshita S, Sato T.

Significance of development of late potentials after anterior wall acute myocaradial infarction despite successful primary angioplasty of the left anterior descending coronary artery. Am J Cardiol 81, 1239-1241 (1998).

Eto K, Takeshita S, Ochiai M, Ozaki Y, Sato T, Isshiki T.

Platelet aggregation in acute coronary syndromes: use of a new aggregometer with laser light scattering to assess platelet aggregability. Cardiovas Res 40, 223-229 (1998b).

Eto K, Takeshita S, Ochiai M, Ozaki Y, Sato T, Isshiki T.

Platelet aggregation in acute coronary syndromes: use of a new aggregometer with laser light scattering to assess platelet aggregability. Cardiovas Res 40, 223-229 (1998b).

Eto K, Hashimoto K, Nakaya H.

Preferential inhibition of IKr by MCI-154, a putative Ca2+sensitizer, in guinea pig atrial cells. Cardiovasc Res 38, 685-694 (1998a).

Eto K, Xue Y-X, Hashimoto K.

Effect of a new Ca2+ sensitizer, MCI-154, on ventricular arrhythmia and the membrane ionic currents. Eur J Pharmacol 298, 247-256 (1996).

Ono K, Eto K, Sakamoto A, Masaki T, Shibata K, Sada T, Hashimoto K, Tsujimoto G.

A negative chronotropic effect of endothelin-1 mediated through ET A receptor in the guinea-pig atria. Circ Res 76, 284-292 (1995).

Ono K, Tsujimoto G, Sakamoto A, Eto K, Masaki T, Ozaki Y, Satake M.

Endothelin-A receptor mediates cardiac inhibition by regulating calcium and potassium currents. Nature 370, 301-304 (1994).
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