Research Activities

Research Activities

Home › Research Activities › Principal Investigators › Jun Takahashi (Professor)

Principal Investigators

Dept. of Clinical Application 
Jun Takahashi (Professor)

Publications

Samata B, Doi D, Nishimura K, Kikuchi T, Watanabe A, Sakamoto Y, Kakuta J, Ono Y, Takahashi J.

Purification of functional human ES and iPSC-derived midbrain dopaminergic progenitors using LRTM1. Nat Commun. 7:13097 (2016)

Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H.

MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells. Sci Rep. 6:32532 (2016)

Nishimura K and Takahashi J.

Drug discovery toward successful cell transplantation therapy for Parkinson's disease using human pluripotent stem cells. Adv Regen Biol. 3: 31772 (2016)

Mishima T, Ishikawa T, Imamura K, Kondo T, Koshiba Y, Takahashi R, Takahashi J, Watanabe A, Fujii N, Tsuboi Y, Inoue H.

Cytoplasmic Aggregates of dynactin in iPSC-derived tyrosine hydroxylase-positive neurons from a patient with Perry syndrome. Park Relat Disord. 30: 67-72 (2016)

Katsukawa M, Nakajima Y, Fukumoto A, Doi D, Takahashi J.

Fail-safe therapy by gamma-ray irradiation against tumor formation by human induced pluripotent stem cell-derived neural progenitors. Stem Cells Dev. 25(11): 815-825 (2016)

Nishimura K, Doi D, Samata B, Murayama S, Tahara T, Onoe H, Takahashi J.

Estradiol facilitates functional integration of induced pluripotent stem cell-derived dopaminergic neurons into striatal neuronal circuits via activation of integrin α5β1. Stem Cell Reports. 6(4): 511-524 (2016)

Motono M, Ioroi Y, Ogura T, Takahashi J.

WNT-C59, a small molecule WNT inhibitor, efficiently induces anterior cortex that includes cortical motor neurons from human pluripotent stem cells. Stem Cells Transl Med. 5(4):552-560 (2016)

Sakaguchi H, Kadoshima T, Soen M, Narii N, Ishida Y, Ohgushi M, Takahashi J, Eiraku M, and Sasai Y.

Generation of functional hippocampal neurons from self-organizing human embryonic stem cell-derived dorsomedial telencephalic tissue. Nat Commun. 6:8896 (2015)

Nishimura K, Murayama S, Takahashi J.

Identification of Neurexophilin 3 as a novel supportive factor for survival of induced pluripotent stem cell-derived dopaminergic progenitors. Stem Cell Trans Med. 4(8): 932-944 (2015)

Yoshida M, Kitaoka S, Egawa N, Yamane M, Ikeda R, Tsukita K, Amano N, Watanabe A, Morimoto M, Takahashi J, Hosoi H, Nakahata T, Inoue H, Saito MK.

Modeling the Early Phenotype at the Neuromuscular Junction of Spinal Muscular Atrophy Using Patient-Derived iPSCs. Stem Cell Reports. 4(4):561-568 (2015)

Fujita M, Hatta T, Sawai T, Takahashi J.

Risk of tumorigenesis and patient hope. AJOB Neurosci. 6(1): 69-70 (2015)

Samata B, Kikuchi T, Miyawaki Y, Morizane A, Mashimo T, Nakagawa M, Okita K, Takahashi J.

X-linked severe combined immunodeficiency (X-SCID) rats for xeno-transplantation and behavioral evaluation. J Neurosci Methods. 243: 68-77 (2015)

Doi D, Samata B, Katsukawa M, Kikuchi T, Morizane A, Ono Y, Sekiguchi K, Nakagawa M, Parmar M, Takahashi J.

Isolation of Human Induced Pluripotent Stem Cell-derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation. Stem Cell Rep 2(3): 337-350 (2014)

Nakagawa M, Taniguchi Y, Senda S, Takizawa N, Ichisaka T, Asano K, Morizane A, Doi D, Takahashi J, Nishizawa M, Yoshida Y, Toyoda T, Osafune K, Sekiguchi K, Yamanaka S.

A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Sci Rep 4: 3594 (2014)

Koyanagi-Aoi M, Ohnuki M, Takahashi K, Okita K, Noma H, Sawamura Y, Teramoto I, Narita M, Sato Y, Ichisaka T, Amano N, Watanabe A, Morizane A, Yamada Y, Sato T, Takahashi J, Yamanaka S.

Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells. Proc Natl Acad Sci U S A. 110(51): 20569-20574 (2013)

Morizane A, Doi D, Kikuchi T, Okita K, Hotta A, Kawasaki T, Hayashi T, Onoe H, Shiina T, Yamanaka S, Takahashi J.

Direct comparison of autologous and allogeneic transplantation of iPSC-derived neural cells in the brain of a nonhuman primate. Stem Cell Reports. 1(4): 283-292 (2013)

Morizane A, Doi D, Takahashi J

Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture. Methods Mol Biol. 1018:11-19 (2013)

Nishimura K, Takahashi J.

Therapeutic application of stem cell technology toward the treatment of Parkinson's disease. Biol Pharm Bull. 36(2) 171-175 (2013)

Yoshikawa T, Samata B, Ogura A, Miyamoto S, Takahashi J.

Systemic administration of valproic acid and zonisamide promotes differentiation of induced pluripotent stem cell-derived dopaminergic neurons. Front Cell Neurosci. published online. (2013)

Ogura A, Morizane A, Nakajima Y, Miyamoto S, Takahashi J.

γ-Secretase inhibitors prevent overgrowth of transplanted neural progenitors derived from human induced pluripotent stem cells. Stem Cells Dev. 22(3): 374-382 (2013)

Egawa N, Kitaoka S, Tsukita K, Naitoh M, Takahashi K, Yamamoto T, Adachi F, Kondo T, Okita K, Asaka I, Aoi T, Watanabe A, Yamada Y, Morizane A, Takahashi J, Ayaki T, Ito H, Yoshikawa K, Yamawaki S, Suzuki S, Watanabe D, Hioki H, Kaneko T, Makioka K, Okamoto K, Takuma H, Tamaoka A, Hasegawa K, Nonaka T, Hasegawa M, Kawata A, Yoshida M, Nakahata T, Takahashi R, Marchetto MCN, Gage FH, Yamanaka S, Inoue H.

Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med. 4: 145-104 (2012)

Gomi M, Takagi Y, Morizane A, Doi D, Nishimura M, Miyamoto S, Takahashi J.

Functional recovery of the murine brain ischemia model using human induced pluripotent stem cell-derived telencephalic progenitors. Brain Res. 1459: 52-60 (2012)

Fujimoto M, Hayashi H , Takagi Y , Hayase M , Marumo T , Gomi M , Nishimura M , Kataoka H , Takahashi J ,Hashimoto N , Nozaki K , Miyamoto S.

Transplantation of telencephalic neural progenitors induced from embryonic stem cells into subacute phase of focal cerebral ischemia. Lab Invest. 92: 522-531 (2012)

Doi D, Morizane A, Kikuchi T, Onoe H, Hayashi T, Kawasaki T, Motono M, Sasai Y, Saiki H, Gomi M, Yoshikawa T, Hayashi H, Shinoyama M, Mohamed R, Suemori H, Miyamoto S, Takahashi J*

Prolonged maturation culture favors a reduction in the tumorigenicity and the dopaminergic function of human ESC-derived neural cells in a primate model of Parkinson's disease Stem Cells. 30(5): 935-945 (2012)

Kitamura A, Fujita Y, Oishi N, Kalaria RN, Washida K, Maki T, Okamoto Y, Hase Y, Yamada M, Takahashi J, Ito H, Tomimoto H, Fukuyama H, Takahashi R, Ihara M.

Selective white matter abnormalities in a novel rat model of vascular dementia Neurobiol Aging, 33(5):1012.e25-35 (2012)

Shinoyama M, Ideguchi M, Hayashi H, Doi D, Hashimoto N, Suzuki M, Takahashi J*

CTLA4-Ig promotes neuronal differentiation in the grafts of embryonic stem cell-derived neural precursor cells Neuroscience 202: 484-491 (2012)

Kikuchi T, Morizane A, Doi D, Onoe H, Hayashi T, Kawasaki T, Saiki H, Miyamoto S, Takahashi J*.

Survival of human induced pluripotent stem cell–derived midbrain dopaminergic neurons in the brain of a primate model of Parkinson's disease. J Parkinson's Disease 1(4):395-412 (2011)

Gomi M, Aoki T, Takagi Y, Nishimura M, Ohsugi Y, Mihara M, Nozaki K, Hashimoto N, Miyamoto S, Takahashi J*.

Single and local blockade of IL-6 signaling promotes neuronal differentiation of transplanted ES cell-derived neural precursor cells. J Neurosci Res 89(9): 1388-1399 (2011)

Okita K, Matsumura Y , Sato Y, Okada A, Morizane A, Okamoto S, Hong Hyenjong, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S.

A more efficient method to generate integration-free human iPS cells. Nat Methods, 8(5): 409-412 (2011)

Morizane A, Doi D, Kikuchi T, Nishimura K, Takahashi J*.

Small molecule inhibitors of BMP and Activin/Nodal signals promote highly efficient neural induction from human pluripotent stem cells. J Neurosci Res 89(2): 117-126 (2011)

Washida K, Ihara M, Nishio K, Fujita Y, Maki T, Yamada M, Takahashi J, Wu X, Kihara T, Ito H, Tomimoto H, Takahashi R.

Nonhypotensive dose of Telmisartan attenuates cognitive impairment partially due to peroxisome proliferator-activated receptor-g activation in mice with chronic cerebral hypoperfusion. Stroke 41: 1798-1806 (2010)

Saiki H, Hayashi T, Takahashi R, Takahashi J*.

Objective and quantitative evaluation of motor function in a monkey model of Parkinson's disease. Neurosci Methods 190(2): 198-204 (2010)

Morizane A and Takahashi J*.

Embryonic stem cell transplantation for the treatment of Parkinson's disease. in Perspectives of Stem Cells H. Ulrich (Ed.) Springer, pp245-254 (2010)

Uemura M, Refaat M. M, Shinoyama M, Hayashi H, Hashimoto N, Takahashi J*.

Matrigel supports survival and neuronal differentiation of grafted embryonic stem cell-derived neural precursor cells. J Neurosci Res 88(3): 542-551 (2010)

Takahashi J*, Takagi Y, Saiki H.

Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys. Methods Mol Biol 482: 199-212 (2009)

Wataya T, Ando S, Muguruma K, Ikeda H, Watanabe K, Eiraku M, Kawada M, Takahashi J, Hashimoto N, Sasai Y.

Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. Proc Natl Acad Sci USA 105(33): 11796-11801 (2008)

Ideguchi M, Shinoyama M, Gomi M, Hayashi H, Hashimoto N, Takahashi J*.

Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells. J Neurosci Res 86(9): 1936-1943 (2008)

Hayashi H, Morizane A, Koyanagi M, Ono Y, Sasai Y, Hashimoto N, Takahashi J*.

Meningeal cells induce dopaminergic neurons from embryonic stem cells. Eur J Neurosci 27: 261-268 (2008)

Koyanagi M, Takahashi J*, Arakawa Y, Doi D, Fukuda H, Hayashi H, Narumiya S, Hashimoto N.

Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J Neurosci Res 86(2): 270-280 (2008)

Takahashi J*.

Stem cell therapy for Parkinson's disease. Expert Rev Neurother 7: 667-675 (2007)

Ono Y, Nakatani T, Sakamoto Y, Mizuhara E, Minaki Y, Kumai M, Hamaguchi A, Nishimura M, Inoue Y, Hayashi H, Takahashi J, Imai T.

Differences in neurogenic potential in floor plate cells along an antero-posterior location: midbrain dopaminergic neurons originate from mesencephalic floor plate cells. Development 134(17): 3213-3225 (2007)

Watanabe K, Ueno M, Kamiya D, Nishiyama A, Matsumura M, Wataya T, Takahashi J, Nishikawa S, Nishikawa S-I, Muguruma K, Sasai Y.

A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotech 25: 681-686 (2007)

Brederlau A, Correia AS, Anisimov SV, Elmi M, Roybon L, Paul G, Morizane A, Bergquist F, Riebe I, Nannmark U, Carta M, Hanse E, Takahashi J, Sasai Y, Funa K, Brundin P, Eriksson PS, Li JY.

Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: effect of in vitro differentiation on graft survival and teratoma formation. Stem Cells 24: 1433-1440 (2006)

Hayashi J, Takagi Y, Fukuda H, Imazato T, Nishimura M, Fujimoto M, Takahashi J, Hashimoto N, Nozaki K.

Primate embryonic stem cell-derived neural progenitors transplanted into ischemic brain. J Cereb Blood Flow Metab. 26: 906-914 (2006)

Morizane A, Takahashi J*, Shinoyama M, Ideguchi M, Takagi Y, Fukuda H, Koyanagi M, Sasai Y, Hashimoto N.

Generation of graftable progenitors of dopaminergic neurons from mouse ES cells by a combination of co-culture and neurosphere methods. J Neurosci Res. 83: 1015-1027 (2006)

Fukuda H, Takahashi J*, Watanabe K, Hayashi H, Morizane A, Koyanagi M, Sasai Y, Hashimoto N.

FACS-based purification of ES cell-derived neural precursors averts tumor formation after transplantation. Stem Cells 24: 763-771 (2006)

Takagi Y, Nishimura M, Morizane A, Takahashi J, Nozaki K, Hayashi J, Hashimoto N.

Survival and differentiation of neural progenitor cells derived from embryonic stem cells and transplanted into ischemic brain. J Neurosurg. 103: 304-310 (2005)

Takagi Y and Takahashi J*.

Primate embryonic stem cells as a source of dopaminergic neurons: a novel transplantation for Parkinson's disease. Disc Med. 5:219-223 (2005)

Fukuda H and Takahashi J*.

Embryonic stem cells as a cell source for treating Parkinson's disease. Expert Opin Biol Ther. 5: 1273-1280 (2005)

Kishi Y, Takahashi J*, Koyanagi M, Morizane A, Okamoto Y, Horiguchi S, Tashiro K, Honjo T, Fujii S, Hashimoto N.

Estrogen promotes differentiation and survival of dopaminergic neurons derived from human neural stem cells. J Neurosci Res. 79(3): 279-286 (2005)

Takagi Y, Takahashi J*, Saiki H, Morizane A, Hayashi T, Kishi Y, Fukuda H, Okamoto Y, Koyanagi M, Ideguchi M, Hayashi H, Imazato T, Kawasaki H, Suemori H, Omachi S, Iida H, Itoh N, Nakatsuji N, Sasai Y, Hashimoto N.

Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model. J Clin Invest. 115(1): 102-109 (2005)

Horiguchi S, Takahashi J*, Kishi Y, Morizane A, Okamoto Y, Koyanagi M, Tsuji M, Tashiro K, Honjo T, Fujii S, Hashimoto N.

Neural precursor cells derived from human embryonic brain retain regional specificity. J Neurosci Res. 75(6):817-824 (2004)

Toda H, Tsuji M, Nakano I, Kobuke K, Hayashi T, Kasahara H, Takahashi J, Mizoguchi A, Houtani T, Sugimoto T, Hashimoto N, Palmer TD, Honjo T, Tashiro K.

Stem cell-derived neural stem/progenitor cell supporting factor (SDNSF) is an autocrine/paracrine survival factor for neural stem/progenitor cells. J Biol Chem. 278(32): 35491-35500 (2003)

Morizane A, Takahashi J*, Takagi Y, Sasai Y, Hashimoto N.

Optimal conditions for in vivo induction of dopaminergic neurons from ES cells through stromal cell-derived inducing activity. J Neurosci Res 69(6):934-939 (2002)

Toda H, Takahashi J*, Iwakami N, Kimura T, Hoki S, Mozumi-Kitamura K, Ono S, Hashimoto N.

Grafting neural stem cells improved the impaired spatial recognition in ischemic rats. Neurosci Lett 316:9-12 (2001)

Wu S, Suzuki Y, Kitada M, Kitaura M, Kataoka K, Takahashi J, Ide C, Nishimira Y.

Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord. Neurosci Lett 312:173-176 (2001)

Tanigaki K, Nozaki F, Takahashi J, Tashiro K, Kurooka H, Honjo T.

Notch1 and Notch3 instructively restrict bFGF-responsive multipotent neural progenitor cells to an astroglial fate. Neuron 29:45-55 (2001)

Nishida A, Takahashi M, Tanihara H, Nakano I, Takahashi J, Mizoguchi A, Ide C, Honda Y.

Incorporation and differentiation of hippocampus-derived neural stem cells transplanted in injured adult rat retina. Invest Ophthalmol Vis Sci. 41:4268-4274 (2000).

Toda H, Takahashi J*, Mizoguchi A, Koyano K, Hashimoto N.

Neurons generated from adult rat hippocampal stem cells form functional glutamatergic and GABAergic synapses in vitro. Exp Neurol. 165:66-76 (2000).

Takagi Y, Nozaki K, Takahashi J, Yodoi J, Ishikawa M, Hashimoto N.

Proliferation of neuronal precursor cells in the dentate gyrus is accelerated after transient forebrain ischemia in mice. Brain Res. 831:283-287 (1999).

Takahashi J, Palmer TD, Gage FH.

Retinoic acid and neurotrophins collaborate to regulate neurogenesis in adult-derived neural stem cell cultures. J. Neurobiol. 38:65-81 (1999).

Takahashi M, Takahashi J, Palmer TD, Gage FH.

Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina. Mol. Cell. Neurosci. 12(6):340-348 (1998).

Palmer TD, Takahashi J, Gage FH.

The adult rat hippocampus contains primordial neural stem cells. Mol. Cell. Neurosci. 8:389-404 (1997).

Nakajima T, Fukamizu A, Takahashi J, Gage FH, Fisher T, Blenis J, Montminy MR.

The signal-dependent coactivator CBP is a nuclear target for pp90RSK. Cell 86:465-474 (1996).

Ray J, Palmer TD, Suhonen J, Takahashi J, Gage FH.

Neurogenesis in the Adult Brain: Lessons Learned from the Studies of Progenitor Cells from the Embryonic and Adult Central Nervous System. in Isolation, Characterization and Utilization of CNS Stem Cells. Gage FH and Christen Y, Eds., Foundation IPSEN, Paris, France. pp 129-149 (1996).

Kojima M, Hoshimaru M, Aoki T, Takahashi J, Otsuka T, Asahi M, Matsuura N, Kikuchi H.

Localization of trkB and low-affinity nerve growth factor receptor mRNA in the developing rat retina. Neurosci. Lett. 205:215-7 (1996)

Koide T, Takahashi J, Hoshimaru M, Kojima M, Otsuka T, Asahi M, Kikuchi H.

Expression of heat shock proteins in the developing rat retina. Neurosci. Lett. 185:183-6 (1995)

Takahashi J, Hoshimaru M, Kikuchi H, Hatanaka M.

Developmental expression of trkB and low-affinity NGF receptor in the rat retina. Neurosci. Lett. 151:174-177 (1993)

Takahashi J, Hoshimaru M, Kikuchi H, Hatanaka M.

Extension of optic nerve fibers on genetically modified cells producing brain-derived neurotrophic factor. Neurosci. Lett. 149:83-86 (1993)

Takahashi J, Hoshimaru M, Kikuchi H, Hatanaka M.

Possible activity of acidic fibroblast growth factor as a progression factor rather than a transforming factor. Biochem. Biophys. Res. Commun. 189:398-405 (1992)
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