Research Activities

Research Activities

Home › Research Activities › Principal Investigators › Hirohide Saito (Professor)

Principal Investigators

Dept. of Life Science Frontiers 
Hirohide Saito (Professor)

Publications

Kashida, Shunnichi, and Hirohide Saito.

Design of Ligand‐Controlled Genetic Switches Based on RNA Interference. Synthetic Biology: Parts, Devices and Applications. 8 (2018).

Endo, Kei, and Hirohide Saito.

mRNA Engineering for the Control of Mammalian Cells in Medical Applications. Applied RNA Bioscience. Springer. Singapore, 2018. 95-114.

Parr Callum, Shinya Yamanaka, Hirohide Saito.*

An update on stem cell biology and engineering for brain development. Molecular Psychiatry. volume 22, pages 808-819 (2017), doi:10.1038/mp.2017.66.

Shibata, T., Fujita, Y., Ohno, H., Suzuki, Y., Hayashi, K., Komatsu, K.R., Kawasaki, S., Hidaka, K., Yonehara, S., Sugiyama, H., Endo, M., Saito, H.*

Protein-Driven RNA Nanostructured Devices That Function in Vitro and Control Mammalian Cell Fate. Nature Communications. 2017 Sep 14;8(1):540. doi: 10.1038/s41467-017-00459-x.

Hirosawa, M., Fujita, Y., Parr, C.J.C., Hayashi, K., Kashida, S., Hotta, A., Woltjen, K., Saito, H.*

Cell-Type-Specific Genome Editing with a microRNA-Responsive CRISPR-Cas9 Switch. Nucleic Acids Research. 2017 Jul 27;45(13):e118. doi: 10.1093/nar/gkx309.

Kawasaki, S., Fujita, Y., Nagaike, T., Tomita, K., Saito, H.*

Synthetic mRNA Devices That Detect Endogenous Proteins and Distinguish Mammalian Cells. Nucleic Acids Research. 2017 Jul 7;45(12):e117. doi: 10.1093/nar/gkx298.

Nakanishi H, Miki K, Komatsu KR, Umeda M, Mochizuki M, Inagaki A, Yoshida Y, Saito H*.

Monitoring and visualizing microRNA dynamics during live cell differentiation using microRNA-responsive non-viral reporter vectors. Biomaterials. 2017 Jun;128:121-135. doi: 10.1016/j.biomaterials.2017.02.033. PMID:28325684

Karagiannis P, Fujita Y, Saito H*.

RNA-based gene circuits for cell regulation. Proc Jpn Acad Ser B Phys Biol Sci. 2016;92(9):412-422.PMID: 27840389

Parr CJ, Katayama S, Miki K, Kuang Y, Yoshida Y, Morizane A, Takahashi J, Yamanaka S, Saito H*.

MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells. Scientific Reports. 2016 Sep 9;6:32532. doi: 10.1038/srep32532.PMID: 27608814

Ohno H, Saito H*.

RNA and RNP as Building Blocks for Nanotechnology and Synthetic Biology. Prog Mol Biol Transl Sci. 2016;139:165-85. doi: 10.1016/bs.pmbts.2015.12.004. Review. PMID: 26970194

Endo K, Hayashi K, Saito H*.

High-resolution Identification and Separation of Living Cell Types by Multiple microRNA-responsive Synthetic mRNAs Scientific Reports. 2016 Feb 23;6:21991. PMID: 26902536

Liliana Wroblewska, Tasuku Kitada, Kei Endo, Velia Siciliano, Breanna Stillo, Hirohide Hirohide Saito*, and Ron Weiss*.

Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery. Nature Biotechnology, 2015, 33(8):839-41. doi: 10.1038/nbt.3301. PMID: 26237515

Miki K, Endo K, Takahashi S, Funakoshi S, Takei I, Katayama S, Toyoda T, Kotaka M, Takaki T, Umeda M, Okubo C, Nishikawa M, Oishi A, Narita M, Miyashita I, Asano K, Hayashi K, Osafune K, Yamanaka S, Saito H*, Yoshida Y*.

Efficient Detection and Purification of Cell Populations Using Synthetic MicroRNA Switches. Cell Stem Cell. 2015 May 20. pii: S1934-5909(15)00163-0. doi: 10.1016/j.stem.2015.04.005. [Epub ahead of print] PMID: 26004781

Shibata T, Suzuki Y, Sugiyama H, Endo M, Saito H*.

Folding RNA-Protein Complex into Designed Nanostructures. Methods Mol Biol. 2015;1316:169-79. doi: 10.1007/978-1-4939-2730-2_14. PMID: 25967061

Ohno H, Osada E, Saito H*.

Design, Assembly, and Evaluation of RNA-Protein Nanostructures. Methods Mol Biol. 2015;1297:197-211. doi: 10.1007/978-1-4939-2562-9_14. PMID: 25896005

Saito H*.

Synthetic RNA technologies to control functions of mammalian cells. Yakugaku Zasshi. 2015;135(3):399-404. doi: 10.1248/yakushi.14-00240-4. Japanese. PMID: 25759049

Kei Endo, Parr Callum, Hirohide Saito*.

Expanding the synthetic ribonucleoprotein world in cells. Nature Methods. News and Views, 11:1105-06 (2014)

Eriko Osada, Yuki Suzuki, Kumi Hidaka, Hirohisa Ohno, Hiroshi Sugiyama, Masayuki Endo, *Hirohide Saito.

Engineering RNA-Protein Complexes with Different Shapes for Imaging and Therapeutic Applications. ACS Nano. 2014 Aug 5. [Epub ahead of print] PMID: 25058166.

*Masami Hagiya, Akihiko Konagaya, Satoshi Kobayashi, Hirohide Saito, Satoshi Murata.

Molecular robots with sensors and intelligence. Acc Chem Res. 2014 Jun 17;47(6):1681-90. doi:10.1021/ar400318d. Epub 2014 Jun 6. PMID: 24905779.

Hirohide Saito.

RNA-protein nanotechnology & synthetic biology. Seikagaku. 2014 Feb;86(1):81-5. Review. Japanese. PMID: 24693704.

Shunnichi Kashida, *Hirohide Saito.

A three-dimensional design strategy for a protein-responsive shRNA switch. Methods Mol Biol. 2014;1111:269-86. doi: 10.1007/978-1-62703-755-6_20.PMID: 24549627

Kei Endo, *Hirohide Saito.

Engineering protein-responsive mRNA switch in mammalian cells. Methods Mol Biol. 2014;1111:183-96. doi: 10.1007/978-1-62703-755-6_13.PMID: 24549620

Kei Endo, Karin Hayashi, *Tan Inoue, and *Hirohide Saito.

A versatile cis-acting inverter module for synthetic translational switches. Nature Communications, 4:2393, DOI: 10.1038/ncomms3393 (2013)

Kei Endo, James A. Stapleton, Karin Hayashi, *Hirohide Saito, and *Tan Inoue.

Quantitative and stimulataneous translational control of distinct mammalian mRNAs. Nucleic Acid Res., 41(13):e135 (2013)

Hirohisa Ohno, Eriko Osada, Tan Inoue, and *Hirohide Saito.

Synthetic RNA-Protein Nanostructures and Their Potential Applications. Review: RNA Nanotechnology and Therapeutics, (ed. Guo, P., CRC press), in press (2013)

Tomoaki Hara, *Hirohide Saito, and *Tan Inoue.

Directed evolution of a synthetic RNA-protein module to create a new translational switch. Chemical Communications (Camb). 2013 Feb 4. [Epub ahead of print], (Selected as the front cover image)

Shunnichi Kashida, *Tan Inoue, and *Hirohide Saito.

Three-dimensionally designed protein-responsive RNA devices for cell signaling regulation. Nucleic Acids Research, 40(18):9369-9378 (Selected as Featured Articles represent the top 5% of papers) (2012)

James A. Stapleton, Kei Endo, Yoshihiko Fujita, Karin Hayashi, Masahiro Takinoue, *Hirohide Saito, and *Tan Inoue.

Feedback Control of Protein Expression in Mammalian Cells by Tunable Synthetic Translational Inhibition. ACS Synthetic Biology, 1(3): 83-88 (2012)

Rei Ohmori, Hirohide Saito, Yoshiya Ikawa, Yoshihiko Fujita, Tan Inoue.

Self-Replication Reactions Dependent on Tertiary Interaction Motifs in an RNA Ligase Ribozyme. J Mol Evol., 73:221-9 (2011)

Sergii Rudiuk, Hirohide Saito, Tomoaki Hara, Tan Inoue, Kenichi Yoshikawa, Damien Baigl.

Light-Regulated mRNA Condensation by a Photosensitive Surfactant Works as a Series Photoswitch of Translation Activity in the Presence of Small RNAs. Biomacromolecules, 12(11):3945-51 (2011)

*Hirohide Saito, Yoshihiko Fujita, Shunnichi Kashida, Karin Hayashi, and *Tan Inoue.

Synthetic human cell fate regulation by protein-driven RNA swiches Nature Communications, 2, 160 (2011)

Hirohisa Ohno, Tetsuhiro Kobayashi, Rinko Kabata, Kei Endo, Takuma Iwasa, Shige Yoshimura, Kunio Takeyasu, *Tan Inoue, and *Hirohide Saito.

Synthetic RNA-protein complex shaped like an equilateral triangle. Nature Nanotechnology, 6: 116-120 (2011)

*Hirohide Saito, Tetsuhiro Kobayashi, Tomoaki Hara, Yoshihiko Fujita, Karin Hayashi, Rie Furushima, and *Tan Inoue.

Synthetic Translational Regulation by an L7Ae-Kink-turn RNP Switch. Nature Chemical Biology, 6: 71-78 (2010)

Shigeyoshi Matsumura, Rei Ohmori, Hirohide Saito, Yoshiya Ikawa, Tan Inoue.

Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction. loop-receptor interaction FEBS Letters, 583 (17): 2819-26 (2009)

André Estévez-Torres, Cécile Crozatier, Antoine Diguet, Tomoaki Hara, Hirohide Saito, Kenichi Yoshikawa, Damien Baigl.

Sequence-independent and reversible photocontrol of transcription/expression systems using a photosensitive nucleic acid binder. PNAS, 106 (30): 12219-23 (2009)

*Hirohide Saito, Yusho Kato, MAel Le Berre, Ayako Yamada, Tan Inoue, Kenichi Yoshikawa, and Damien Baigl.

Time-resolved tracking of a minimum gene expression system reconstituted in giant liposomes. ChemBioChem. 10 (10): 1640-3 (2009)

*Hirohide Saito and *Tan Inoue.

Synthetic Biology with RNA motifs. Int. J. Biochem. Cell Biol., 41(2):398-404 (2009).

Tomoaki Shiohara, *Hirohide Saito and Tan Inoue.

A designed RNA selection: establishment of a stable complex between a target and selectant RNA via two coordinated interactions. Nucleic Acids Res. 37(3):e23 (2009).

Hirohide Saito, Tamiko Minamisawa, Takao Yamori, Kiyotaka Shiba.

Motif-programmed artificial protein induces apoptosis in several cancer cells by disrupting mitochondria. Cancer Science, 99 (2): 398-406. (2008)

*Hirohide Saito, Shunichi Kashida, Tan Inoue, Kiyotaka Shiba.

The role of peptide motifs in the evolution of a protein network. Nucleic Acid. Res., 35 (19): 6357-6366. (2007)

*Hirohide Saito, Tan Inoue.

RNA and RNP as new molecular parts in synthetic biology. J. Biotechnol., 132 (1): 1-7. (2007)

Hirohide Saito, Tamiko Minamisawa, Kiyotaka Shiba.

Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs. Nucleic Acid. Res., 35(6):e38 (2007)

Hirohide Saito, Takako Honma,, Tamiko Minamisawa, Kanami Yamazaki, Tetsuo Noda, Takao Yamori, Kiyotaka Shiba.

Synthesis of functional proteins by mixing peptide motifs. Chemistry & Biology 11(6): 765-773 (2004)

Krishna Ramaswamy, Hirohide Saito, Hiroshi Murakami, Kiyotaka Shiba, Hiroaki Suga.

Designer Ribozymes: Programming the tRNA Specificity into Flexizyme. J. Am. Chem. Soc., 126 (37) 11454 –11455 (2004)

Hiroshi Murakami, Hirohide Saito, Hiroaki Suga.

A versatile tRNA aminoacylation catalyst based on RNA. Chemistry & Biology 10(7): 655-662 (2003)

Hirohide Saito, Hiroaki Suga.

Outersphere and innersphere coordinated metal ions in an aminoacyl-tRNA synthetase ribozyme. Nucleic Acid. Res., 30 (23): 5151-5159 (2002)

Hirohide Saito, Hiroaki Suga.

A ribozyme exclusively aminoacylates the 3´- hydroxyl group of the tRNA terminal adenosine. J. Am. Chem. Soc., 123 (29): 7178-7179 (2001)

Hirohide Saito, Kimitsuna Watanabe, Hiroaki Suga.

Concurrent molecular recognition of both the amino acid and tRNA by a ribozyme. RNA, 7 (12): 1867-1878 (2001)

Hirohide Saito, Dimitrios Kourouklis, Hiroaki Suga.

An in vitro evolved precursor tRNA with aminoacylation activity. The EMBO Journal, 20 (7): 1797-1806 (2001)
Authored Book Chapters

Hiroaki Suga, Hirohide Saito, Hodgson David

"RNA-catalyzed tRNA aminoacylation" The Aminoacyl-tRNA Synthetases, Landes Bioscience, 364-373 (2004)
go top