研究活動
Research Activities
研究活動
Research Activities
主任研究者
Principal Investigators
臨床応用研究部門 櫻井 英俊 准教授
発表論文
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Takenaka-Ninagawa N, Goto M, Yoshioka CKB, Miki M, Sakurai H
- Cell Therapy for Duchenne Muscular Dystrophy Using Induced Pluripotent Stem Cell-derived Muscle Stem Cells and the Potential of Regenerative Rehabilitation Current Opinion in Biomedical Engineering, 2024 Feb 30:100523
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Kita Y, Okuzaki Y, Naoe Y, Lee J, Bang U, Okawa N, Ichiki A, Jonouchi T, Sakurai H, Kojima Y, Hotta A
- Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs Stem Cell Reports, 2023 Aug 18(9):1753-1765.100523
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Otomo J, Woltjen K, Sakurai H
- Uniform transgene activation in Tet-On systems depends on sustained rtTA expression iScience, 2023 Aug 26(10):107685
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Rashid MI, Ito T, Miya F, Shimojo D, Arimoto K, Onodera K, Okada R, Nagashima T, Yamamoto K, Khatun Z, Shimul RI, Niwa JI, Katsuno M, Sobue G, Okano H, Sakurai H, Shimizu K, Doyu M, Okada Y
- Simple and efficient differentiation of human iPSCs into contractible skeletal muscles for muscular disease modeling Scientific Reports, 2023 May 13(1):8146
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Namatame I, Ishii K, Shin T, Shimojo D, Yamagishi Y, Asano H, Kishimoto Y, Fuse H, Nishi Y, Sakurai H, Nakahata T, Sasaki-Iwaoka H
- Screening Station, a novel laboratory automation system for physiologically relevant cell-based assays SLAS Technology, 2023 Apr 28(5):351-360
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Sato M, Goto M, Yamanouchi K, Sakurai H
- A new immunodeficient Duchenne muscular dystrophy rat model to evaluate engraftment after human cell transplantation Frontiers in Physiology, 2023 Apr 10;14:1094359.
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Kawada R, Jonouchi T, Kagita A, Sato M, Hotta A, Sakurai H.
- Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs Scientific Reports, 2023 Jan 11;13(1):94.
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Nalbandian M, Zhao M, Sakurai H.
- Evaluation of hiPSC-Derived Muscle Progenitor Cell Transplantation in a Mouse Duchenne Muscular Dystrophy Model Methods in Moleular Biology, 2023;2587:527-536.
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Mitsuru Sasaki-Honda
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Implications From Experiences as a Skeletal Muscle Disease Patient With Physical Handicaps in the Experimental Biology Filed
筋ジストロフィー患者の経験から: ──運動障害・実験系研究 TRENDS IN THE SCIENCES 学術の動向, 27 (10):10_10-10_14 -
Zhang S, Ohkawara B, Ito M, Huang Z, Zhao F, Nakata T, Takeuchi T, Sakurai H, Komaki H, Kamon M, Araki T, Ohno K.
- A mutation in DOK7 in congenital myasthenic syndrome forms aggresome in cultured cells, and reduces DOK7 expression and MuSK phosphorylation in patient-derived iPS cells Human Molecular Genetics, 2023 Apr 20;32(9):1511-1523.
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Kamiya D, Takenaka-Ninagawa N, Motoike S, Kajiya M, Akaboshi T, Zhao C, Shibata M, Senda S, Toyooka Y, Sakurai H, Kurihara H, Ikeya M.
- Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage npj Regenerative Medicine, 2022 Sep 15;7(1):47.
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Honda M, Makino T, Zhao X, Matsuto M, Sakurai H, Takahashi Y, Shimizu M, Sato R, Yamauchi Y.
- Pathophysiological levels of GDF11 activate Smad2/Smad3 signaling and induce muscle atrophy in human iPSC-derived myocytes American Journal of Physiology Cell Physiolosy, 2022 Nov 1;323(5):C1402-C1409.
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Ashida Y, Himori K, Tokuda N, Naito A, Yamauchi N, Takenaka-Ninagawa N, Aoki Y, Sakurai H, Yamada T
- Dissociation of SH3 and cysteine rich domain 3 and junctophilin 1 from dihydropyridine receptor in dystrophin-deficient muscles American Journal of Physiology Cell Physiolosy, 2022 Sep 1;323(3):C885-C895.
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Nakamori M, Shimizu H, Ogawa K, Hasuike Y, Nakajima T, Sakurai H, Araki T, Okada Y, Kakita A, Mochizuki H
- Cell type-specific abnormalities of central nervous system in myotonic dystrophy type 1 Brain Communications, 2022 Jun 10;4(3):fcac154.
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Fujiwara K, Yamamoto R, Kubota T, Tazumi A, Sabuta T, Takahashi MP, Sakurai H.
- Mature Myotubes Generated From Human-Induced Pluripotent Stem Cells Without Forced Gene Expression Frontiers in Cell and Developmental Biology, 2022 May 30;10:886879.
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Nalbandian M, Zhao M, Kato H, Jonouchi T, Nakajima-Koyama M, Yamamoto T, Sakurai H
- Single-cell RNA-seq reveals heterogeneity in hiPSC-derived muscle progenitors and E2F family as a key regulator of proliferation Life Science Alliance, 2022 Apr 22;5(8):e202101312.
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Miura Y, Sato M, Kuwahara T, Ebata T, Tabata Y, Sakurai H
- Transplantation of human iPSC-derived muscle stem cells in the diaphragm of Duchenne muscular dystrophy model mice PLoS ONE, 2022 Apr 4;17(4):e0266391. doi: 10.1371/journal.pone.0266391.
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Fujiwara K, Yamamoto R, Kubota T, Tazumi A, Sabuta T, Takahashi MP, Sakurai H
- Mature myotubes generated from human induced pluripotent stem cells without forced gene expression Frontiers in Cell and Developmental Biology, 2022 May 10:886879 doi:10.3389/fcell.2022.886879
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Nalbandian, M., Zhao, M., Kato, M., Jonouchi, T., Nakajima-Koyama, M., Yamamoto, T and Sakurai, H.
- Single-cell RNAseq reveals heterogeneity in hiPSC-MuPCs and E2F as a key regulator of proliferation Life Science Alliance, 2022 Apr.22 5 e02101312
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Miura Y, Sato M, Kuwahara T, Ebata T, Tabata Y, Sakurai H
- Transplantation of human iPSC-derived muscle stem cells in the diaphragm of Duchenne muscular dystrophy model mice PLOS ONE, 2022 Apr 4;17(4):e0266391. doi: 10.1371/journal.pone.0266391.
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Harada A, Goto M, Kato A, Takenaka-Ninagawa N, Tanaka A, Noguchi S, Ikeya M, Sakurai H.
- Systemic Supplementation of Collagen VI by Neonatal Transplantation of iPSC-Derived MSCs Improves Histological Phenotype and Function of Col6-Deficient Model Mice. Front Cell Dev Biol., 2021 Nov 23;9:790341. doi: 10.3389/fcell.2021.790341. eCollection 2021. PMID: 34888314
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Tomoya Uchimura, Hidetoshi Sakurai
- Orai1-STIM1 regulates increased Ca2+ mobilization, leading to contractile Duchenne muscular dystrophy phenotypes in patient-derived induced pluripotent stem cells. Biomedicines., 2021 Oct 31;9(11):1589. doi: 10.3390/biomedicines9111589.
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Takenaka-Ninagawa N, Kim J, Zhao M, Sato M, Jonouchi T, Goto M, Yoshioka CKB, Ikeda R, Harada A, Sato T, Ikeya M, Uezumi A, Nakatani M, Noguchi S, Sakurai H.
- Collagen-VI supplementation by cell transplantation improves muscle regeneration in Ullrich congenital muscular dystrophy model mice. Stem Cell Res Ther. 2021 Aug 9;12(1):446. doi: 10.1186/s13287-021-02514-3. PMID: 34372931
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Uchimura T, Asano T, Nakata T, Hotta A, Sakurai H
- A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs Cell Reports Medicine, 2021 Jun 15 2(6):100298
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Minas Nalbandian, Mingming Zhao, Mitsuru Sasaki-Honda, Tatsuya Jonouchi, Antonio Lucena-Cacace, Takuma Mizusawa, Masahiko Yasuda, Yoshinori Yoshida, Akitsu Hotta, and Hidetoshi Sakurai
- Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy Stem Cell Reports, 13;16(4):883-898 (2021)
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Sasamata M, Shimojo D, Fuse H, Nishi Y, Sakurai H, Nakahata T, Yamagishi Y, Sasaki-Iwaoka H
- Establishment of a Robust Platform for Induced Pluripotent Stem Cell Research Using Maholo LabDroid SLAS Technology, doi: 10.1177/24726303211000690 (2021)
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Mitsuhashi S, Nakagawa S, Sasaki-Honda M, Sakurai H, Frith MC, Mitsuhashi H
- Nanopore direct RNA sequencing detects DUX4-activated repeats and isoforms in human muscle cells Human Molecular Genetics, 30(7):552-563 (2021)
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Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T
- MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells Scientific Reports, 11(1):1161 (2021)
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Zhao M, Tazumi A, Takayama S, Takenaka-Ninagawa N, Nalbandian M, Nagai M, Nakamura Y, Nakasa M, Watanabe A, Ikeya M, Hotta A, Ito Y, Sato T, Sakurai H
- Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model Stem cell reports, 15(1):80-94 (2020)
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Yoshida T, Jonouchi T, Osafune K, Takita J, Sakurai H
- A Liver Model of Infantile-Onset Pompe Disease Using Patient-Specific Induced Pluripotent Stem Cells Frontiers in Cell and Developmental Biology, 7:316 (2019)
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Takada H, Kaieda A, Tawada M, Nagino T, Sasa K, Oikawa T, Oki A, Sameshima T, Miyamoto K, Miyamoto M, Kokubu Y, Tozawa R, Sakurai H, Saito B
- Identification of 2,6-Disubstituted 3H-Imidazo[4,5-b]pyridines as Therapeutic Agents for Dysferlinopathies through Phenotypic Screening on Patient-Derived Induced Pluripotent Stem Cells Journal of Medical Chemistry, 62(10):9175-9187 (2019)
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Kokubu Y, Nagino T, Sasa K, Oikawa T, Miyake K, Kume A, Fukuda M, Fuse H, Tozawa R, Sakurai H
- Phenotypic drug screening for dysferlinopathy using patient derived-induced pluripotent stem cells STEM CELLS Translational Medicine, 10:1017-1029 (2019)
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Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H
- A Patient-derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-causative DUX4 Human Molecular Genetics. 2018 Aug 9. doi: 10.1093/hmg/ddy293. [Epub ahead of print]
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Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H
- A Patient-derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-causative DUX4 Human Molecular Genetics. 2018 Aug 9. doi: 10.1093/hmg/ddy293. [Epub ahead of print]
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Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C.
- Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports. 2018 Jan 10. pii: S2213-6711(17)30561-1. doi: 10.1016/j.stemcr.2017.12.013.
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Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C.
- Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports. 2018 Jan 10. pii: S2213-6711(17)30561-1. doi: 10.1016/j.stemcr.2017.12.013.
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Lee JJA, Maruyama R, Sakurai H, Yokota T.
- Cell Membrane Repair Assay Using a Two-photon Laser Microscope. J Vis Exp. 2018 Jan 2;(131). doi: 10.3791/56999.
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Uchimura T, Otomo J, Sato M, Sakurai H
- A human iPS cell myogenic differentiation system permitting high-throughput drug screening Stem Cell Research, 2017 Dec;25:98-106. doi: 10.1016/j.scr.2017.10.023. Epub 2017 Oct 28.
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Yoshida T, Awaya T, Jonouchi T, Kimura R, Kimura S, Era T, Heike T, Sakurai H.
- A Skeletal Muscle Model of Infantile-onset Pompe Disease with Patient-specific iPS Cells. Scientific Reports. 2017 Dec;25:98-106. doi: 10.1016/j.scr.2017.10.023. Epub 2017 Oct 28
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Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T.
- Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9. Stem Cells Int. 2017;2017:9210494.
Ueki J, Nakamori M, Nakamura M, Nishikawa M, Yoshida Y, Tanaka A, Morizane A, Kamon M, Araki T, Takahashi M.P., Watanabe A, Inagaki N, Sakurai H.
- Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability. Scientific Reports. 2017 Feb 13;7:42522.
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Horio F, Sakurai H, Ohsawa Y, Nakano S, Matsukura M, Fujii I.
- Functional validation and expression analysis of myotubes converted from skin fibroblasts using a simple direct reprogramming strategy. eNeurologicalSci., 2016 Nov 3;6:9-15. doi: 10.1016/j.ensci.2016.11.002. eCollection 2017 Mar. PMID: 29260008
Iwasaki H, Imamura T, Morino K, Shimosato T, Tawa M, Ugi S, Sakurai H, Maegawa H, Okamura T.
- MicroRNA-494 plays a role in fiber type-specific skeletal myogenesis in human induced pluripotent stem cells. Biochem Biophys Res Commun. 2015 Oct 28. pii: S0006-291X(15)30827-5. doi: 10.1016/j.bbrc.2015.10.128.
Shoji E., Sakurai H., Nishino T., Nakahata T., Heike T., Awaya T., Fujii N., Manabe Y., Matsuo M., Sehara-Fujisawa A.
- Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells. Scientific Reports. 2015 :5, Article number: 12831; doi:10.1038/srep12831.
Li HL., Fujimoto N., Sasakawa N., Shirai S., Ohkame T., Sakuma T., Tanaka M., Amano N., Watanabe A., Sakurai H., Yamamoto T., Yamanaka S., Hotta A.
- Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2015 Jan 13;4(1):143-54.
Yasuno T., Osafune K., Sakurai H., Asaka I., Tanaka A., Yamaguchi S., Yamada K., Hitomi H., Arai S., Kurose Y., Higaki Y., Sudo M., Ando S., Nakashima H., Saito T., Kaneoka H.
- Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency. Biochem Biophys Res Commun. 2014 May 30;448(2):175-81.
Noguchi M., Hosoda K., Nakane M., Mori E., Nakao K., Taura D., Yamamoto Y., Kusakabe T., Sone M., Sakurai H., Fujikura J., Ebihara K., Nakao K.
- In vitro characterization and engraftment of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells. Stem Cells Dev. 2013 Nov 1;22(21):2895-905.
Sakai H., Sato T., Sakurai H., Yamamoto T., Hanaoka K., Montarras D., Sehara-Fujisawa A.
- Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice. PLoS One. 2013 May 9;8(5):e63016.
Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.
- Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Myoshi Myopathy in vitro. PLoS One. 2013, 8(4):e61540.
Sakurai H., Sakaguchi Y., Shoji E., Nishino T., Maki I., Sakai H., Hanaoka K., Kakizuka A., Sehara-Fujisawa A.
- In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem. PLoS ONE. 2012,7(10): e47078.
Inami Y., Yoshikai T., Ito S., Nishio N., Suzuki H., Sakurai H., Isobe K.
- Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype. Immunology and Cell Biology. 2011, 89, 314-21.
Tanaka C, Ito S, Nishio N, Kodera Y, Sakurai H, Suzuki H, Nakao A, Isobe K.
- GADD34 suppresses wound healing by upregulating expression of myosin IIA. Transgenic Res. 2010 Aug;19(4):637-45.
Iida A., Sakaguchi K., Sato K., Sakurai H., Nishimura D., Iwaki A., Takeuchi M., Kobayashi M., Misaki K., Yonemura S., Kawahara A., Sehara-Fujisawa A.
- Metalloprotease-Dependent Onset of Blood Circulation in Zebrafish. Current Biology. 2010 June; 20(12): 1110-16.
Sakurai H., Inami Y., Tamamura Y., Yoshikai T., Sehara-Fujisawa A., Isobe K.
- Bidirectional induction toward paraxial mesodermal derivatives from mouse ES cells in chemically defined medium. Stem Cell Research. 2009 Sep-Nov;(2-3): 157-69.
Sakurai H., Okawa Y., Inami Y., Nishio N., Isobe K.
- Paraxial mesodermal progenitors derived from mouse embryonic stem cells contribute to muscle regeneration via differentiation into muscle satellite cells. Stem Cells. 2008 Jul;26(7): 1865-73.
Nishio N., Okawa Y., Sakurai H., Isobe K.
- Neutrophil depletion delays wound repair in aged mice. Age. 2008; 30: 11-19.
Sakurai H., Era T., Lakt LM., Okada M., Nakai S., Nishikawa S., Nishikawa SI.
- In vitro modeling of paraxial and lateral mesoderm differentiation reveals early reversibility. Stem Cells. 2006 Mar;24(3): 575-86.
Tada S., Era T., Furusawa C., Sakurai H., Nishikawa S., Kinoshita M., Chiba T., Nishikawa SI.
- Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture. Development. 2005 Oct;132(19): 4363-74.
Shoji E, Woltjen K, Sakurai H.
- Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells. Methods in Mol Biol. 2015 May 14.
庄子栄美、櫻井英俊
- ヒトiPS細胞から骨格筋細胞への効率的な分化誘導 ES・iPS細胞実験スタンダード(羊土社) 2014:194-201
櫻井 英俊
- iPS細胞を使った筋疾患研究 病理と臨床 2015: 33(6): 592-598
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- 患者由来iPS細胞による筋疾患モデル作製 医学のあゆみ 2015: 252(9): 969-975
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- 三好型ミオパチー 遺伝子医学MOOK 2015: "27 iPS細胞を用いた難病研究": 71-76
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- iPS細胞を用いた難治性筋疾患治療研究 実験医学2014: 32(9):1359-1365
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- iPS細胞創薬は10年以内に実現するか?「Yes」の立場から Frontiers in Parkinson Disease 2014: 7(4): 200-203
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- iPS細胞を用いた筋疾患モデル 最新医学 2014: 69: 589-597
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- iPS細胞技術を用いた筋再生療法の開発 整形・災害外科 2013: 56(5):689-694
竹中 菜々、櫻井 英俊
- iPS細胞を用いた筋ジストロフィー症の病態解析 Medical Science Digest 2013: 39(11): 514-517
竹中 菜々、櫻井 英俊
- iPS細胞を使う-骨格筋の研究へ- 整形外科 2013: 64(11): 1221-1223
櫻井 英俊、山中 伸弥
- iPS細胞 先進医療 NAVIGATOR 2013: 93-95
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- 多能性幹細胞による筋ジストロフィー治療 生体の科学(医学書院) 2011: 62(2), 161-164
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- iPS細胞(人工多能性幹細胞研究)研究 日本臨床 2011: 69(12): 2114-18