研究活動
Research Activities

研究活動
Research Activities

Home › 研究活動 › 主任研究者 › 櫻井 英俊 准教授

主任研究者
Principal Investigators

臨床応用研究部門 櫻井 英俊 准教授

発表論文

Fujiwara K, Yamamoto R, Kubota T, Tazumi A, Sabuta T, Takahashi MP, Sakurai H

Mature myotubes generated from human induced pluripotent stem cells without forced gene expression Frontiers in Cell and Developmental Biology, 2022 May 10:886879 doi:10.3389/fcell.2022.886879

Nalbandian, M., Zhao, M., Kato, M., Jonouchi, T., Nakajima-Koyama, M., Yamamoto, T and Sakurai, H.

Single-cell RNAseq reveals heterogeneity in hiPSC-MuPCs and E2F as a key regulator of proliferation Life Science Alliance, 2022 Apr.22 5 e02101312

Miura Y, Sato M, Kuwahara T, Ebata T, Tabata Y, Sakurai H

Transplantation of human iPSC-derived muscle stem cells in the diaphragm of Duchenne muscular dystrophy model mice PLOS ONE, 2022 Apr 4;17(4):e0266391. doi: 10.1371/journal.pone.0266391.

Harada A, Goto M, Kato A, Takenaka-Ninagawa N, Tanaka A, Noguchi S, Ikeya M, Sakurai H.

Systemic Supplementation of Collagen VI by Neonatal Transplantation of iPSC-Derived MSCs Improves Histological Phenotype and Function of Col6-Deficient Model Mice. Front Cell Dev Biol., 2021 Nov 23;9:790341. doi: 10.3389/fcell.2021.790341. eCollection 2021. PMID: 34888314

Tomoya Uchimura, Hidetoshi Sakurai

Orai1-STIM1 regulates increased Ca2+ mobilization, leading to contractile Duchenne muscular dystrophy phenotypes in patient-derived induced pluripotent stem cells. Biomedicines., 2021 Oct 31;9(11):1589. doi: 10.3390/biomedicines9111589.

Takenaka-Ninagawa N, Kim J, Zhao M, Sato M, Jonouchi T, Goto M, Yoshioka CKB, Ikeda R, Harada A, Sato T, Ikeya M, Uezumi A, Nakatani M, Noguchi S, Sakurai H.

Collagen-VI supplementation by cell transplantation improves muscle regeneration in Ullrich congenital muscular dystrophy model mice. Stem Cell Res Ther. 2021 Aug 9;12(1):446. doi: 10.1186/s13287-021-02514-3. PMID: 34372931

Uchimura T, Asano T, Nakata T, Hotta A, Sakurai H

A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs Cell Reports Medicine, 2021 Jun 15 2(6):100298

Minas Nalbandian, Mingming Zhao, Mitsuru Sasaki-Honda, Tatsuya Jonouchi, Antonio Lucena-Cacace, Takuma Mizusawa, Masahiko Yasuda, Yoshinori Yoshida, Akitsu Hotta, and Hidetoshi Sakurai

Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy Stem Cell Reports, 13;16(4):883-898 (2021)

Sasamata M, Shimojo D, Fuse H, Nishi Y, Sakurai H, Nakahata T, Yamagishi Y, Sasaki-Iwaoka H

Establishment of a Robust Platform for Induced Pluripotent Stem Cell Research Using Maholo LabDroid SLAS Technology, doi: 10.1177/24726303211000690 (2021)

Mitsuhashi S, Nakagawa S, Sasaki-Honda M, Sakurai H, Frith MC, Mitsuhashi H

Nanopore direct RNA sequencing detects DUX4-activated repeats and isoforms in human muscle cells Human Molecular Genetics, 30(7):552-563 (2021)

Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T

MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells Scientific Reports, 11(1):1161 (2021)

Zhao M, Tazumi A, Takayama S, Takenaka-Ninagawa N, Nalbandian M, Nagai M, Nakamura Y, Nakasa M, Watanabe A, Ikeya M, Hotta A, Ito Y, Sato T, Sakurai H

Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model Stem cell reports, 15(1):80-94 (2020)

Yoshida T, Jonouchi T, Osafune K, Takita J, Sakurai H

A Liver Model of Infantile-Onset Pompe Disease Using Patient-Specific Induced Pluripotent Stem Cells Frontiers in Cell and Developmental Biology, 7:316 (2019)

Takada H, Kaieda A, Tawada M, Nagino T, Sasa K, Oikawa T, Oki A, Sameshima T, Miyamoto K, Miyamoto M, Kokubu Y, Tozawa R, Sakurai H, Saito B

Identification of 2,6-Disubstituted 3H-Imidazo[4,5-b]pyridines as Therapeutic Agents for Dysferlinopathies through Phenotypic Screening on Patient-Derived Induced Pluripotent Stem Cells Journal of Medical Chemistry, 62(10):9175-9187 (2019)

Kokubu Y, Nagino T, Sasa K, Oikawa T, Miyake K, Kume A, Fukuda M, Fuse H, Tozawa R, Sakurai H

Phenotypic drug screening for dysferlinopathy using patient derived-induced pluripotent stem cells STEM CELLS Translational Medicine, 10:1017-1029 (2019)

Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H

A Patient-derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-causative DUX4 Human Molecular Genetics. 2018 Aug 9. doi: 10.1093/hmg/ddy293. [Epub ahead of print]

Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H

A Patient-derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-causative DUX4 Human Molecular Genetics. 2018 Aug 9. doi: 10.1093/hmg/ddy293. [Epub ahead of print]

Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C.

Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports. 2018 Jan 10. pii: S2213-6711(17)30561-1. doi: 10.1016/j.stemcr.2017.12.013.

Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C.

Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports. 2018 Jan 10. pii: S2213-6711(17)30561-1. doi: 10.1016/j.stemcr.2017.12.013.

Lee JJA, Maruyama R, Sakurai H, Yokota T.

Cell Membrane Repair Assay Using a Two-photon Laser Microscope. J Vis Exp. 2018 Jan 2;(131). doi: 10.3791/56999.

Uchimura T, Otomo J, Sato M, Sakurai H

A human iPS cell myogenic differentiation system permitting high-throughput drug screening Stem Cell Research, 2017 Dec;25:98-106. doi: 10.1016/j.scr.2017.10.023. Epub 2017 Oct 28.

Yoshida T, Awaya T, Jonouchi T, Kimura R, Kimura S, Era T, Heike T, Sakurai H.

A Skeletal Muscle Model of Infantile-onset Pompe Disease with Patient-specific iPS Cells. Scientific Reports. 2017 Dec;25:98-106. doi: 10.1016/j.scr.2017.10.023. Epub 2017 Oct 28

Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T.

Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9. Stem Cells Int. 2017;2017:9210494.

Ueki J, Nakamori M, Nakamura M, Nishikawa M, Yoshida Y, Tanaka A, Morizane A, Kamon M, Araki T, Takahashi M.P., Watanabe A, Inagaki N, Sakurai H.

Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability. Scientific Reports. 2017 Feb 13;7:42522.

Horio F, Sakurai H, Ohsawa Y, Nakano S, Matsukura M, Fujii I.

Functional validation and expression analysis of myotubes converted from skin fibroblasts using a simple direct reprogramming strategy. eNeurologicalSci., 2016 Nov 3;6:9-15. doi: 10.1016/j.ensci.2016.11.002. eCollection 2017 Mar. PMID: 29260008

Iwasaki H, Imamura T, Morino K, Shimosato T, Tawa M, Ugi S, Sakurai H, Maegawa H, Okamura T.

MicroRNA-494 plays a role in fiber type-specific skeletal myogenesis in human induced pluripotent stem cells. Biochem Biophys Res Commun. 2015 Oct 28. pii: S0006-291X(15)30827-5. doi: 10.1016/j.bbrc.2015.10.128.

Shoji E., Sakurai H., Nishino T., Nakahata T., Heike T., Awaya T., Fujii N., Manabe Y., Matsuo M., Sehara-Fujisawa A.

Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells. Scientific Reports. 2015 :5, Article number: 12831; doi:10.1038/srep12831.

Li HL., Fujimoto N., Sasakawa N., Shirai S., Ohkame T., Sakuma T., Tanaka M., Amano N., Watanabe A., Sakurai H., Yamamoto T., Yamanaka S., Hotta A.

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Stem Cell Reports. 2015 Jan 13;4(1):143-54.

Yasuno T., Osafune K., Sakurai H., Asaka I., Tanaka A., Yamaguchi S., Yamada K., Hitomi H., Arai S., Kurose Y., Higaki Y., Sudo M., Ando S., Nakashima H., Saito T., Kaneoka H.

Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency. Biochem Biophys Res Commun. 2014 May 30;448(2):175-81.

Noguchi M., Hosoda K., Nakane M., Mori E., Nakao K., Taura D., Yamamoto Y., Kusakabe T., Sone M., Sakurai H., Fujikura J., Ebihara K., Nakao K.

In vitro characterization and engraftment of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells. Stem Cells Dev. 2013 Nov 1;22(21):2895-905.

Sakai H., Sato T., Sakurai H., Yamamoto T., Hanaoka K., Montarras D., Sehara-Fujisawa A.

Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice. PLoS One. 2013 May 9;8(5):e63016.

Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.

Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Myoshi Myopathy in vitro. PLoS One. 2013, 8(4):e61540.

Sakurai H., Sakaguchi Y., Shoji E., Nishino T., Maki I., Sakai H., Hanaoka K., Kakizuka A., Sehara-Fujisawa A.

In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem. PLoS ONE. 2012,7(10): e47078.

Inami Y., Yoshikai T., Ito S., Nishio N., Suzuki H., Sakurai H., Isobe K.

Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype. Immunology and Cell Biology. 2011, 89, 314-21.

Tanaka C, Ito S, Nishio N, Kodera Y, Sakurai H, Suzuki H, Nakao A, Isobe K.

GADD34 suppresses wound healing by upregulating expression of myosin IIA. Transgenic Res. 2010 Aug;19(4):637-45.

Iida A., Sakaguchi K., Sato K., Sakurai H., Nishimura D., Iwaki A., Takeuchi M., Kobayashi M., Misaki K., Yonemura S., Kawahara A., Sehara-Fujisawa A.

Metalloprotease-Dependent Onset of Blood Circulation in Zebrafish. Current Biology. 2010 June; 20(12): 1110-16.

Sakurai H., Inami Y., Tamamura Y., Yoshikai T., Sehara-Fujisawa A., Isobe K.

Bidirectional induction toward paraxial mesodermal derivatives from mouse ES cells in chemically defined medium. Stem Cell Research. 2009 Sep-Nov;(2-3): 157-69.

Sakurai H., Okawa Y., Inami Y., Nishio N., Isobe K.

Paraxial mesodermal progenitors derived from mouse embryonic stem cells contribute to muscle regeneration via differentiation into muscle satellite cells. Stem Cells. 2008 Jul;26(7): 1865-73.

Nishio N., Okawa Y., Sakurai H., Isobe K.

Neutrophil depletion delays wound repair in aged mice. Age. 2008; 30: 11-19.

Sakurai H., Era T., Lakt LM., Okada M., Nakai S., Nishikawa S., Nishikawa SI.

In vitro modeling of paraxial and lateral mesoderm differentiation reveals early reversibility. Stem Cells. 2006 Mar;24(3): 575-86.

Tada S., Era T., Furusawa C., Sakurai H., Nishikawa S., Kinoshita M., Chiba T., Nishikawa SI.

Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture. Development. 2005 Oct;132(19): 4363-74.
プロトコール

Shoji E, Woltjen K, Sakurai H.

Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells. Methods in Mol Biol. 2015 May 14.

庄子栄美、櫻井英俊

ヒトiPS細胞から骨格筋細胞への効率的な分化誘導 ES・iPS細胞実験スタンダード(羊土社) 2014:194-201
著作物

櫻井 英俊

iPS細胞を使った筋疾患研究 病理と臨床 2015: 33(6): 592-598

櫻井 英俊

患者由来iPS細胞による筋疾患モデル作製 医学のあゆみ 2015: 252(9): 969-975

櫻井 英俊

三好型ミオパチー 遺伝子医学MOOK 2015: "27 iPS細胞を用いた難病研究": 71-76

櫻井 英俊

iPS細胞を用いた難治性筋疾患治療研究 実験医学2014: 32(9):1359-1365

櫻井 英俊

iPS細胞創薬は10年以内に実現するか?「Yes」の立場から Frontiers in Parkinson Disease 2014: 7(4): 200-203

櫻井 英俊

iPS細胞を用いた筋疾患モデル 最新医学 2014: 69: 589-597

櫻井 英俊

iPS細胞技術を用いた筋再生療法の開発 整形・災害外科 2013: 56(5):689-694

竹中 菜々、櫻井 英俊

iPS細胞を用いた筋ジストロフィー症の病態解析 Medical Science Digest 2013: 39(11): 514-517

竹中 菜々、櫻井 英俊

iPS細胞を使う-骨格筋の研究へ- 整形外科 2013: 64(11): 1221-1223

櫻井 英俊、山中 伸弥

iPS細胞 先進医療 NAVIGATOR 2013: 93-95

櫻井 英俊

多能性幹細胞による筋ジストロフィー治療 生体の科学(医学書院) 2011: 62(2), 161-164

櫻井 英俊、山中 伸弥

iPS細胞(人工多能性幹細胞研究)研究 日本臨床 2011: 69(12): 2114-18
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